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无根藤(Cassytha filiformis)系樟科无根藤属(Cassytha)寄生缠绕草本植物,又名无娘藤、无爷藤、罗网藤等,主要分布于热带和亚热带地区,在我国主产于海南、广西、台湾、贵州以及福建等地区[1]。无根藤为我国民间常用药用植物,常用于糖尿病、风湿关节炎、肾炎水肿以及尿路结石等多种疾病的治疗[2-3]。无根藤的药用成分丰富,包括阿朴菲类生物碱、黄酮及挥发油类等[4]。目前的研究表明,阿朴菲类生物碱是无根藤的特征成分,从无根藤中分离得到30余个阿朴菲类生物碱,主要包括4种结构类型:阿朴菲类生物碱、氧化阿朴菲类生物碱、原阿朴菲类生物碱和吗啡烷类生物碱[5]。近年来,随着药理学和分子生物学的发展,研究人员陆续发现,阿朴菲类生物碱在抗糖尿病方面展示了较好的活性[6-7]。目前,阿朴菲类生物碱在抗糖尿病方面取得一定的研究成果,展示良好的应用前景。但是无根藤来源阿朴菲类生物碱的研究主要集中在抗肿瘤及抗寄生虫方面[8],而在抗糖尿病的研究较少。
为了进一步探究无根藤中阿朴菲类生物碱成分,笔者所在的课题组通过酸溶碱沉和乙酸乙酯萃取法得到无根藤总生物碱浸膏,进一步运用硅胶柱色谱、葡聚糖凝胶、制备薄层色谱法和半制备高效液相色谱等多种色谱方法进行分离纯化,利用核磁共振波谱和高分辨质谱等技术进行结构鉴定。同时,采用葡萄糖氧化酶法评价了所得化合物对HL-7702细胞葡萄糖消耗活性,挖掘无根藤中具有降糖活性的阿朴菲类生物碱,旨在为该植物资源的开发利用提供科学依据。
Analysis of aporphine alkaloids in Cassytha filiformis
doi: 10.15886/j.cnki.rdswxb.20230043
- Received Date: 2023-03-28
- Accepted Date: 2023-06-06
- Rev Recd Date: 2023-05-23
- Available Online: 2024-03-20
- Publish Date: 2024-03-25
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Key words:
- Cassytha filiformis /
- aporphine alkaloids /
- extraction-separation /
- spectral analysis /
- glucose consumption activity
Abstract: In order to discover aporphine alkaloids with hypoglycemic activity in Cassytha filiformis, the total alkaloids were extracted from C. filiformis by acid solubilization and alkaline precipitation, obtained by ethyl acetate extraction, separated and purified by various chromatographic methods. The structures of the compounds purified were identified by using spectrometry, and the glucose oxidase method was used to evaluate the glucose consumption activity of the resulting aporphine alkaloids on HL-7702 cells. Thirteen aporphine alkaloids were identified from the extract of C. filiformis: cassamedine ( 1 ), cassameridine ( 2 ), thalicminine ( 3 ), neolitsine ( 4 ), N-metilseconeolitsine ( 5 ), N-methyl-2, 3, 6-trimethoxymorphin-andien-7-one ( 6 ), 1, 2-methylenedioxy-3, 10, 11-trimethoxyaporphine ( 7 ), cassythidine ( 8 ), norneolitsine ( 9 ), O-methylcassythine ( 10 ), cassythine ( 11 ), filiformine ( 12 ), and 1, 2-Dimethoxy-5, 6, 6a, 7-tetrahydro-4H-benzo[de][1, 3]benzodioxolo[56-g]quinoline ( 13 ). Among them, compounds 5 and 13 were first isolated from C. filiformis. Cellular glucose consumption activity assay showed that compounds 3 and 11 significantly promoted glucose consumption in HL-7702 cells and displayed a potential value for research and development into hypoglycemic lead compounds.
Citation: | HUANG Zibao, CHEN Mimi, ZHANG Yong, ZHANG Xiaopo, DONG Lin, ZHANG Caiyun. Analysis of aporphine alkaloids in Cassytha filiformis[J]. Journal of Tropical Biology, 2024, 15(2): 251-258. doi: 10.15886/j.cnki.rdswxb.20230043 |