Molecular Detection of Sugarcane Ratoon Stunting Disease in Sugarcane Producing Areas of China

SHEN Linbo; WU Nannan; FENG Xiaoyan; WANG Wenzhi; QIONG Guoru; ZHAO Tingting; ZHANG Shuzhen

doi:10.15886/j.cnki.rdswxb.2019.04.002

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In order to clarify the occurrence of sugarcane ratoon stunting disease（RSD） in sugarcane plantations in China, 598 sugarcane leaf samples were collected from 12 sugarcane plantations in the main sugarcane producing areas like Guangxi, Yunnan, Guangdong and Hainan in China, and the pathogen of RSD was identified by using specific primers through polymerase chain reaction（PCR）. The results showed that 99 out of 598 sugarcane samples were detected to contain bacteria of RSD, with an average detection rate of 16.56%. RSD detection rates varied in 12 sugarcane plantations, the highest in Lincang sugarcane plantations（27.78%） and the lowest in Nanning sugarcane plantations（4.88%）. RSD was detected in 15 main sugarcane cultivars, with positive detection rates ranging from 15.38% to 44.44%. Among the sugarcane cultivars, sugarcane cultivar ROC 22 was most seriously infected with RSD, and its RSD positive detection rate was 44.44%, while sugarcane cultivar Haitang 22 was less infected with RSD and its RSD positive detection rate was 15.38%. It can be seen that sugarcane ratoon stunting disease is common in sugarcane cultivars in China.

Molecular Detection of Sugarcane Ratoon Stunting Disease in Sugarcane Producing Areas of China

1. Sugarcane Research Center, Chinese Academy of Tropical Agricultural Sciences/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Ministry of Agriculture and Rural Affairs Key Laboratory of Tropical Crop Biology and Genetic Resources, Haikou, Hainan 571101, China;

2. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

Received Date: 2019-04-18

Rev Recd Date: 2019-10-08

Key words:

Abstract: In order to clarify the occurrence of sugarcane ratoon stunting disease（RSD） in sugarcane plantations in China, 598 sugarcane leaf samples were collected from 12 sugarcane plantations in the main sugarcane producing areas like Guangxi, Yunnan, Guangdong and Hainan in China, and the pathogen of RSD was identified by using specific primers through polymerase chain reaction（PCR）. The results showed that 99 out of 598 sugarcane samples were detected to contain bacteria of RSD, with an average detection rate of 16.56%. RSD detection rates varied in 12 sugarcane plantations, the highest in Lincang sugarcane plantations（27.78%） and the lowest in Nanning sugarcane plantations（4.88%）. RSD was detected in 15 main sugarcane cultivars, with positive detection rates ranging from 15.38% to 44.44%. Among the sugarcane cultivars, sugarcane cultivar ROC 22 was most seriously infected with RSD, and its RSD positive detection rate was 44.44%, while sugarcane cultivar Haitang 22 was less infected with RSD and its RSD positive detection rate was 15.38%. It can be seen that sugarcane ratoon stunting disease is common in sugarcane cultivars in China.

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Reference (23)

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Molecular Detection of Sugarcane Ratoon Stunting Disease in Sugarcane Producing Areas of China

doi: 10.15886/j.cnki.rdswxb.2019.04.002

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通讯作者: 陈斌, bchen63@163.com

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