Analysis of Sulfonamides Residues in Seawater of Dongzhai Harbor, Hainan Based on Ultra Performance Liquid Chromatography-tandem Mass Spectrometry

ZHANG Tengyun; XU Wen; ZHANG Mingshan; ZHAO Hongwei

doi:10.15886/j.cnki.rdswxb.2019.02.016

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Volume 10 Issue 2

Jun. 2019

Turn off MathJax

Article Contents

Article Navigation > Journal of Tropical Biology > 2019 > 10(2): 197-203

ZHANG Tengyun, XU Wen, ZHANG Mingshan, ZHAO Hongwei. Analysis of Sulfonamides Residues in Seawater of Dongzhai Harbor, Hainan Based on Ultra Performance Liquid Chromatography-tandem Mass Spectrometry[J]. Journal of Tropical Biology, 2019, 10(2): 197-203. doi: 10.15886/j.cnki.rdswxb.2019.02.016

Citation:

ZHANG Tengyun, XU Wen, ZHANG Mingshan, ZHAO Hongwei. Analysis of Sulfonamides Residues in Seawater of Dongzhai Harbor, Hainan Based on Ultra Performance Liquid Chromatography-tandem Mass Spectrometry[J]. Journal of Tropical Biology, 2019, 10(2): 197-203. doi: 10.15886/j.cnki.rdswxb.2019.02.016

Analysis of Sulfonamides Residues in Seawater of Dongzhai Harbor, Hainan Based on Ultra Performance Liquid Chromatography-tandem Mass Spectrometry

doi: 10.15886/j.cnki.rdswxb.2019.02.016

1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China;
2. Hainan Academy of Environmental Science & Hainan Environmental Monitoring Center, Haikou, Hainan 570100, China;
3. State Key Laboratory of Utilization of Marine Resources in South China Sea, Hainan University, Haikou, Hainan 570228, China

Received Date: 2019-01-25
Rev Recd Date: 2019-03-02

Abstract

A method for simultaneous detection of 13 sulfonamides drugs in seawater by using ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） was established and optimized, and used for analysis of the surface seawater samples collected from Dongzhai Harbour, Hainan. The results show that in case of the peak types of sulfonamides in the chromatogram are better and the correlation coefficient of the standard curve is high（R²≥ 0.996） when the sulfaonamides in the sea water were determined by using UPLC-MS/MS with mobile phase A: methanol-acetonitrile（V∶V=1∶1） and mobile phase B: 0.1% formic acid-10 mM ammonium acetate as the mobile phase and the initial mobile phase（5% mobile phase A and 95% mobile phase B） as the reconstituted solvent. Thirteen sulfonamides were separated well in 14 minutes and trheir recovery rates were from 70.4 to 103%, with a relative standard deviation（RSD） of between 1.2% and 12.8%. This method had high sensitivity and good reproducibility, and is suitable for simultaneous detection of sulfonamides in seawater. At the same time, five sulfonamides were detected in the water samples of the Dongzhai Harbour. The detection rates were from 60% to 100%. The highest detection frequency of sulfamethoxazole（SMX） was 100%, sulfameter（SME） and sulfachloropyridazine（SCP） had high detection rates（around 70%） in the Dongzhai Harbor, and the other two detected were sulfamethazine（SMA） and sulfadiazine（SDZ）. The highest concentration of sulfonamides detected was up to 3.35 ng·L^-1. Compared with other areas, the concentrations of sulfonamides detected in Dongzhai Harbor was at a relatively low level.
- Ultra performance liquid chromatography-tandem mass spectrometry,
- Dongzhai Harbour,
- surface seawater,
- sulfonamides

References

[1]	BOGIALLI S, CURINI R, Corcia A D, et al.A liquid chromatography mass spectrometry assay for analyzing sulfonamide antibacterials in cattle and fish muscle tissues[J].Analytical Chemistry, 2003, 75 (8):1798-1804.
[2]	MANAGAKI S, MURATA A, TAKADA H, et al.Distribution of macrolides, sulfonamides, and trimethoprim in tropical waters:ubiquitous occurrence of veterinary antibiotics in the Mekong Delta[J].Environmental Science and Technology, 2008, 41 (23):8004-8010.
[3]	SARMAH A K, MEYER M T, BOXALL A B A.A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment[J].Chemosphere, 2006, 65 (5):725-759.
[4]	MERMIN J, LULE J, EKWARU J P, et al.Effect of co-trimoxazole prophylaxis on morbidity, mortality, CD4-cell count, and viral load in HIV infection in rural Uganda[J].The Lancet, 2004, 364 (9443):1428-1434.
[5]	CARR A, TINDALL B, BREW B J, et al.Low-dose trimethoprim-sulfamethoxazole prophylaxis for toxoplasmic encephalitis in patients with AIDS.[J].Annals of Internal Medicine, 1992, 117 (2):106-1011.
[6]	KÜMMERER K.Antibiotics in the aquatic environment-a review-part I [J].Chemosphere, 2009, 75 (4):417-434.
[7]	ZHAO S, LIU X, CHENG D, et al.Temporal-spatial variation and partitioning prediction of antibiotics in surface water and sediments from the intertidal zones of the Yellow River Delta, China[J].Science of The Total Environment, 2016:S004896971631405X.
[8]	ZHANG R, ZHANG G, ZHENG Q, et al.Occurrence and risks of antibiotics in the Laizhou Bay, China:Impacts of river discharge[J].Ecotoxicology & Environmental Safety, 2012, 80:208-215.
[9]	ZOU S, XU W, ZHANG R, et al.Occurrence and distribution of antibiotics in coastal water of the Bohai Bay, China:Impacts of river discharge and aquaculture activities[J].Environmental Pollution, 2011, 159 (10):2913-2920.
[10]	CHENG D, XIE Y, YU Y, et al.Occurrence and Partitioning of Antibiotics in the Water Column and Bottom Sediments from the Intertidal Zone in the Bohai Bay, China[J].Wetlands, 2016, 36 (1):167-179.
[11]	LIU K, YIN X, ZHANG D, et al.Distribution, sources, and ecological risk assessment of quinotone antibiotics in the surface sediments from Jiaozhou Bay wetland, China[J].Marine Pollution Bulletin, 2018.129 (2):859-865.
[12]	ZHENG Q, ZHANG R, WANG Y, et al.Occurrence and distribution of antibiotics in the Beibu Gulf, China:Impacts of river discharge and aquaculture activities[J].Marine Environmental Research, 2012, 78:26-33.
[13]	ZHANG R, TANG J, LI J, et al.Occurrence and risks of antibiotics in the coastal aquatic environment of the Yellow Sea, North China[J].Science of The Total Environment, 2013, 450/451:197-204.
[14]	MENGELERS M J B, KLETER G A, HOOGENBOOM L A P, et al.The biotransformation of sulfadimethoxine, sulfadimidine, sulfamethoxazole, trimethoprim and aditoprim by primary cultures of pig hepatocytes[J].Journal of Veterinary Pharmacology and Therapeutics, 1997, 20 (1):9.
[15]	符致德, 周宁, 吴翔宇, 等.海南东寨港水生动物资源状况及渔业发展对策[J].南方农业, 2018, 12 (28):87-91.
[16]	JIA A, HU J, WU X, et al.Occurrence and source apportionment of sulfonamides and their metabolites in Liaodong Bay and the adjacent Liao River basin, North China[J].Environmental toxicology and chemistry / SETAC, 2011, 30 (6):1252-1260.
[17]	ZHANG X, ZHANG D, ZHANG H, et al.Occurrence, distribution, and seasonal variation of estrogenic compounds and antibiotic residues in Jiulongjiang River, South China[J].Environmental Science and Pollution Research, 2012, 19 (5):1392-1404.
[18]	TANG J, SHI T, WU X, et al.The occurrence and distribution of antibiotics in Lake Chaohu, China:Seasonal variation, potential source and risk assessment[J].Chemosphere, 2015, 122:154-161.
[19]	王海东, 王弋平.普外科手术预防使用抗生素的现状调查 (附海南省25间医院普外科手术病例抽查报告) [J].海南医学, 2006, 17 (12):119-120.
[20]	韩静磊, 贺德春, 王志良, 等.规模化养殖场废水中抗生素种类及残留特征研究[J].广州化学, 2012, 37 (1):27-31.
[21]	赵文, 潘运舟, 兰天, 等.海南商品有机肥中重金属和抗生素含量状况与分析[J].环境化学, 2017, 36 (2):408-419.

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(43) PDF downloads(0) Cited by()

Proportional views

Analysis of Sulfonamides Residues in Seawater of Dongzhai Harbor, Hainan Based on Ultra Performance Liquid Chromatography-tandem Mass Spectrometry

1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China;

2. Hainan Academy of Environmental Science & Hainan Environmental Monitoring Center, Haikou, Hainan 570100, China;

3. State Key Laboratory of Utilization of Marine Resources in South China Sea, Hainan University, Haikou, Hainan 570228, China

Received Date: 2019-01-25

Rev Recd Date: 2019-03-02

Key words:

Ultra performance liquid chromatography-tandem mass spectrometry /
Dongzhai Harbour /
surface seawater /
sulfonamides

Abstract: A method for simultaneous detection of 13 sulfonamides drugs in seawater by using ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） was established and optimized, and used for analysis of the surface seawater samples collected from Dongzhai Harbour, Hainan. The results show that in case of the peak types of sulfonamides in the chromatogram are better and the correlation coefficient of the standard curve is high（R²≥ 0.996） when the sulfaonamides in the sea water were determined by using UPLC-MS/MS with mobile phase A: methanol-acetonitrile（V∶V=1∶1） and mobile phase B: 0.1% formic acid-10 mM ammonium acetate as the mobile phase and the initial mobile phase（5% mobile phase A and 95% mobile phase B） as the reconstituted solvent. Thirteen sulfonamides were separated well in 14 minutes and trheir recovery rates were from 70.4 to 103%, with a relative standard deviation（RSD） of between 1.2% and 12.8%. This method had high sensitivity and good reproducibility, and is suitable for simultaneous detection of sulfonamides in seawater. At the same time, five sulfonamides were detected in the water samples of the Dongzhai Harbour. The detection rates were from 60% to 100%. The highest detection frequency of sulfamethoxazole（SMX） was 100%, sulfameter（SME） and sulfachloropyridazine（SCP） had high detection rates（around 70%） in the Dongzhai Harbor, and the other two detected were sulfamethazine（SMA） and sulfadiazine（SDZ）. The highest concentration of sulfonamides detected was up to 3.35 ng·L^-1. Compared with other areas, the concentrations of sulfonamides detected in Dongzhai Harbor was at a relatively low level.

HTML

Reference (21)

[1]	BOGIALLI S, CURINI R, Corcia A D, et al.A liquid chromatography mass spectrometry assay for analyzing sulfonamide antibacterials in cattle and fish muscle tissues[J].Analytical Chemistry, 2003, 75 (8):1798-1804.
[2]	MANAGAKI S, MURATA A, TAKADA H, et al.Distribution of macrolides, sulfonamides, and trimethoprim in tropical waters:ubiquitous occurrence of veterinary antibiotics in the Mekong Delta[J].Environmental Science and Technology, 2008, 41 (23):8004-8010.
[3]	SARMAH A K, MEYER M T, BOXALL A B A.A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment[J].Chemosphere, 2006, 65 (5):725-759.
[4]	MERMIN J, LULE J, EKWARU J P, et al.Effect of co-trimoxazole prophylaxis on morbidity, mortality, CD4-cell count, and viral load in HIV infection in rural Uganda[J].The Lancet, 2004, 364 (9443):1428-1434.
[5]	CARR A, TINDALL B, BREW B J, et al.Low-dose trimethoprim-sulfamethoxazole prophylaxis for toxoplasmic encephalitis in patients with AIDS.[J].Annals of Internal Medicine, 1992, 117 (2):106-1011.
[6]	KÜMMERER K.Antibiotics in the aquatic environment-a review-part I [J].Chemosphere, 2009, 75 (4):417-434.
[7]	ZHAO S, LIU X, CHENG D, et al.Temporal-spatial variation and partitioning prediction of antibiotics in surface water and sediments from the intertidal zones of the Yellow River Delta, China[J].Science of The Total Environment, 2016:S004896971631405X.
[8]	ZHANG R, ZHANG G, ZHENG Q, et al.Occurrence and risks of antibiotics in the Laizhou Bay, China:Impacts of river discharge[J].Ecotoxicology & Environmental Safety, 2012, 80:208-215.
[9]	ZOU S, XU W, ZHANG R, et al.Occurrence and distribution of antibiotics in coastal water of the Bohai Bay, China:Impacts of river discharge and aquaculture activities[J].Environmental Pollution, 2011, 159 (10):2913-2920.
[10]	CHENG D, XIE Y, YU Y, et al.Occurrence and Partitioning of Antibiotics in the Water Column and Bottom Sediments from the Intertidal Zone in the Bohai Bay, China[J].Wetlands, 2016, 36 (1):167-179.
[11]	LIU K, YIN X, ZHANG D, et al.Distribution, sources, and ecological risk assessment of quinotone antibiotics in the surface sediments from Jiaozhou Bay wetland, China[J].Marine Pollution Bulletin, 2018.129 (2):859-865.
[12]	ZHENG Q, ZHANG R, WANG Y, et al.Occurrence and distribution of antibiotics in the Beibu Gulf, China:Impacts of river discharge and aquaculture activities[J].Marine Environmental Research, 2012, 78:26-33.
[13]	ZHANG R, TANG J, LI J, et al.Occurrence and risks of antibiotics in the coastal aquatic environment of the Yellow Sea, North China[J].Science of The Total Environment, 2013, 450/451:197-204.
[14]	MENGELERS M J B, KLETER G A, HOOGENBOOM L A P, et al.The biotransformation of sulfadimethoxine, sulfadimidine, sulfamethoxazole, trimethoprim and aditoprim by primary cultures of pig hepatocytes[J].Journal of Veterinary Pharmacology and Therapeutics, 1997, 20 (1):9.
[15]	符致德, 周宁, 吴翔宇, 等.海南东寨港水生动物资源状况及渔业发展对策[J].南方农业, 2018, 12 (28):87-91.
[16]	JIA A, HU J, WU X, et al.Occurrence and source apportionment of sulfonamides and their metabolites in Liaodong Bay and the adjacent Liao River basin, North China[J].Environmental toxicology and chemistry / SETAC, 2011, 30 (6):1252-1260.
[17]	ZHANG X, ZHANG D, ZHANG H, et al.Occurrence, distribution, and seasonal variation of estrogenic compounds and antibiotic residues in Jiulongjiang River, South China[J].Environmental Science and Pollution Research, 2012, 19 (5):1392-1404.
[18]	TANG J, SHI T, WU X, et al.The occurrence and distribution of antibiotics in Lake Chaohu, China:Seasonal variation, potential source and risk assessment[J].Chemosphere, 2015, 122:154-161.
[19]	王海东, 王弋平.普外科手术预防使用抗生素的现状调查 (附海南省25间医院普外科手术病例抽查报告) [J].海南医学, 2006, 17 (12):119-120.
[20]	韩静磊, 贺德春, 王志良, 等.规模化养殖场废水中抗生素种类及残留特征研究[J].广州化学, 2012, 37 (1):27-31.
[21]	赵文, 潘运舟, 兰天, 等.海南商品有机肥中重金属和抗生素含量状况与分析[J].环境化学, 2017, 36 (2):408-419.

Message Board

Analysis of Sulfonamides Residues in Seawater of Dongzhai Harbor, Hainan Based on Ultra Performance Liquid Chromatography-tandem Mass Spectrometry

doi: 10.15886/j.cnki.rdswxb.2019.02.016

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Related