Functional Analysis of the mon1 Gene in Fusarium oxysporum f. sp. cubense Race 4

LI Heng; CHANG Wenjun; CHEN Hanqing; QIAO Fan; ZENG Huicai

doi:10.15886/j.cnki.rdswxb.2019.02.005

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Based on the clues obtained from our previous proteomics studies, the mon1 gene from Fusarium oxysporum f.sp. cubense Race 4（Foc4） was found to play a role in pathogenic infection. To further characterize the functions of the mon1 in the infection of Foc4, the mon1 gene was knocked out through homologous recombination method and the phenotype and pathogenicity of the mutant strains analyzed. The results showed that the mutant strains of Foc4 grew slowly, produced less spores and thinner mycelia with fewer branches, and was lower in their resistance to exogenous oxygen stress, their capacity of cell wall penetration and their cellulose utilization, as compared with the wild strains. Pathogenicity test showed the mutant strains exhibited a significantly low infection against the banana seedlings. All the results suggested that the mon1 gene played a certain role in the growth, development, sporulation and pathogenicity of the Foc4.

Functional Analysis of the mon1 Gene in Fusarium oxysporum f. sp. cubense Race 4

1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China;

2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural sciences, Key Laboratory of Tropical Crop Biology and Genetic Resources, Ministry of Agriculture and Rural Sciences, Hainan Key Laboratory of Tropical Microbial Resources, Haikou, Hainan 571101, China

Received Date: 2019-03-08

Rev Recd Date: 2019-05-10

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Abstract: Based on the clues obtained from our previous proteomics studies, the mon1 gene from Fusarium oxysporum f.sp. cubense Race 4（Foc4） was found to play a role in pathogenic infection. To further characterize the functions of the mon1 in the infection of Foc4, the mon1 gene was knocked out through homologous recombination method and the phenotype and pathogenicity of the mutant strains analyzed. The results showed that the mutant strains of Foc4 grew slowly, produced less spores and thinner mycelia with fewer branches, and was lower in their resistance to exogenous oxygen stress, their capacity of cell wall penetration and their cellulose utilization, as compared with the wild strains. Pathogenicity test showed the mutant strains exhibited a significantly low infection against the banana seedlings. All the results suggested that the mon1 gene played a certain role in the growth, development, sporulation and pathogenicity of the Foc4.

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Functional Analysis of the mon1 Gene in Fusarium oxysporum f. sp. cubense Race 4

doi: 10.15886/j.cnki.rdswxb.2019.02.005

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