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Volume 10 Issue 2
Jun.  2019
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Article Navigation >  Journal of Tropical Biology  > 2019  >  10(2): 99-105

QIAN Jiang, SUN Zhihua, LIU Yiqiao, XIE Pinxi, ZHANGSUN Dongting, LUO Sulan. Cytotoxicity of α-conotoxin TxID in Lung Cancer[J]. Journal of Tropical Biology, 2019, 10(2): 99-105. doi: 10.15886/j.cnki.rdswxb.2019.02.001
Citation: QIAN Jiang, SUN Zhihua, LIU Yiqiao, XIE Pinxi, ZHANGSUN Dongting, LUO Sulan. Cytotoxicity of α-conotoxin TxID in Lung Cancer[J]. Journal of Tropical Biology, 2019, 10(2): 99-105. doi: 10.15886/j.cnki.rdswxb.2019.02.001
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Cytotoxicity of α-conotoxin TxID in Lung Cancer

doi: 10.15886/j.cnki.rdswxb.2019.02.001

  • College of Life Sciences and Pharmacy Hainan University/Ministry of Education Key Laboratory of Tropical Biological Resources/Haikou Key Laboratory for Marine Drugs,, Haikou, Hainan 570228, China

  • Received Date: 2019-04-03
  • Rev Recd Date: 2019-04-18
  • an attempt was made to study the cytotoxicity effect of α-conotoxin TxID in DMS114 s, a small cell lung cancer cell line. An α-conotoxin TxID was synthesized by solid phase peptide synthesis and two-step oxidation and identified by mass spectrometry and HPLC. The expression of α3 and β4 nAChR subunits in DMS114 cells was detected by PCR. MTT assay was used to detect the cytotoxicity of TxID or Adriamycin（ADM） or TxID combined with ADM in the DMS114 cells. The results showed that α-conotoxin TxID was successfully synthesized, and that α3 and α4 nAChR subunits were detected in DMS114 cells. The α-conotoxin TxID dependently inhibited the proliferation of small cell lung cancer DMS114 cells and HEL normal lung cells, and had higher cytotoxicity effect at some specific concentrations on DMS114 cells than on HEL cells. In addition, combination of TxID and ADM（1∶1） potentiated the cytotoxicity in DMS114 cells. In short, this study demonstrated for the first time that α-conotoxin TxID can inhibit the proliferation of small cell lung cancer DMS114 cells, which could provide guidance for the study of novel anticancer drugs.
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Cytotoxicity of α-conotoxin TxID in Lung Cancer

doi: 10.15886/j.cnki.rdswxb.2019.02.001
  • College of Life Sciences and Pharmacy Hainan University/Ministry of Education Key Laboratory of Tropical Biological Resources/Haikou Key Laboratory for Marine Drugs,, Haikou, Hainan 570228, China
  • Received Date: 2019-04-03
  • Rev Recd Date: 2019-04-18

Abstract: an attempt was made to study the cytotoxicity effect of α-conotoxin TxID in DMS114 s, a small cell lung cancer cell line. An α-conotoxin TxID was synthesized by solid phase peptide synthesis and two-step oxidation and identified by mass spectrometry and HPLC. The expression of α3 and β4 nAChR subunits in DMS114 cells was detected by PCR. MTT assay was used to detect the cytotoxicity of TxID or Adriamycin（ADM） or TxID combined with ADM in the DMS114 cells. The results showed that α-conotoxin TxID was successfully synthesized, and that α3 and α4 nAChR subunits were detected in DMS114 cells. The α-conotoxin TxID dependently inhibited the proliferation of small cell lung cancer DMS114 cells and HEL normal lung cells, and had higher cytotoxicity effect at some specific concentrations on DMS114 cells than on HEL cells. In addition, combination of TxID and ADM（1∶1） potentiated the cytotoxicity in DMS114 cells. In short, this study demonstrated for the first time that α-conotoxin TxID can inhibit the proliferation of small cell lung cancer DMS114 cells, which could provide guidance for the study of novel anticancer drugs.

QIAN Jiang, SUN Zhihua, LIU Yiqiao, XIE Pinxi, ZHANGSUN Dongting, LUO Sulan. Cytotoxicity of α-conotoxin TxID in Lung Cancer[J]. Journal of Tropical Biology, 2019, 10(2): 99-105. doi: 10.15886/j.cnki.rdswxb.2019.02.001
Citation: QIAN Jiang, SUN Zhihua, LIU Yiqiao, XIE Pinxi, ZHANGSUN Dongting, LUO Sulan. Cytotoxicity of α-conotoxin TxID in Lung Cancer[J]. Journal of Tropical Biology, 2019, 10(2): 99-105. doi: 10.15886/j.cnki.rdswxb.2019.02.001
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