Analysis of Chemical Constituents from the Fruiting Bodies of Fomes fomentarius

Key words: 
• Fomes fomentarius /
• chemical constituents /
• biological activities

Abstract: Compounds were isolated and purified from the fruiting bodies of Fomes fomentarius by silica gel,Sephadex LH-20 column chromatography and preparative HPLC method to analyze their biological activities. The structures of the compounds were identified through the combined analysis of physicochemical properties and spectral data. The acetylcholinesterase and α-glucosidase inhibitory activity of compounds were evaluated by Ellman colorimetric method and PNPG method,respectively. Similarly,the anti-oxidative ability of the fruiting bodies of F. fomentarius were determined by the DPPH method,and 96-well plate microanalysis were used to determine their antibacterial activity. Eleven compounds were isolated from the ethyl acetate extract obtained from the fruiting bodies of F. fomentarius and identified as 3,4-dihydroxybenzoic acid ethyl ester（ 1）,3,4-dihydroxybenzaldehyde（ 2）,4-（ 3,4-dihydroxyphenyl）-3E-buten-2-one（ 3）,2,4-dihydroxy-3,5-dimethylacetophenone（ 4）,p-hydroxybenzaldehyde（ 5）,4-hydroxyacetophenone（ 6）,ergosta-7,22-diene-3β-ol（ 7）,ergosta-7,22-diene-3-one（ 8）,4-（ 4-hydroxyphenyl） but-3-en-2-one（ 9）,1,5-Bis-（ 3,4-dihydroxyphenyl）-1,4-pentadien-3-one（ 10）,3,4-dihydroxyacetophenone（ 11）. The compounds 2,10 and 11 showed α-glucosidase inhibitory activity,the compounds 3,4,9-11 showed acetylcholinesterase inhibitory activity,the compounds 1-11 exhibited anti-oxidative ability,and the compound 10 exhibited antimicrobial activity.