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Volume 9 Issue 4
Dec.  2018
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Article Navigation >  Journal of Tropical Biology  > 2018  >  9(4): 393-400

YAN Bingyu, GONG Xiaoxiao, TAN Yurong, WANG Peng, LI Shuangjiang, PAN Yingwen, LIU Jinping. Cloning and Expression Analysis of OnRR9 Gene in Oncidium[J]. Journal of Tropical Biology, 2018, 9(4): 393-400. doi: 10.15886/j.cnki.rdswxb.2018.04.005
Citation: YAN Bingyu, GONG Xiaoxiao, TAN Yurong, WANG Peng, LI Shuangjiang, PAN Yingwen, LIU Jinping. Cloning and Expression Analysis of OnRR9 Gene in Oncidium[J]. Journal of Tropical Biology, 2018, 9(4): 393-400. doi: 10.15886/j.cnki.rdswxb.2018.04.005
shu

Cloning and Expression Analysis of OnRR9 Gene in Oncidium

doi: 10.15886/j.cnki.rdswxb.2018.04.005

  • 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China;

  • 2. Post-Entry Quarantine Station for Tropical Plant, Hainan Entry-Exit Inspection and Quarantine Bureau, Haikou, Hainan 570311, China

  • Received Date: 2018-09-25
  • Rev Recd Date: 2018-09-29
  • A gene of response regulator,OnRR9 gene（ GenBank accession number: MH758789）,was cloned from the petals of Oncidium Gower Ramsey flowers with RACE technology. The OnRR9 gene is 627 bp in length and encodes a protein of 208 amino acids in size. The secondary structure analysis of the protein showed that OnRR9 protein is a hydrophilic secreted protein localized in the nucleus. The homologous sequence alignment revealed that the OnRR9 gene belongs to the YesN/AraC family and contains a conserved REC domain in the amino acid sequence,which may be involved in the two-component signal transduction pathway. The construction of the phylogenetic tree demonstrated that the OnRR9 protein is closely related to the type A ARRs in Arabidopsis thaliana,suggesting that the OnRR9 may be involved in the response of cytokinin signaling. Real-time fluorescence quantitative analysis indicated that the OnRR9 gene had the maximum expression in the full blossom flower but was low expressed before and after full blossom,suggesting that this gene might play a role in the iniitation of petal senescence of Oncidium Gower Ramsey flowers.
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Cloning and Expression Analysis of OnRR9 Gene in Oncidium

doi: 10.15886/j.cnki.rdswxb.2018.04.005
  • 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China;
  • 2. Post-Entry Quarantine Station for Tropical Plant, Hainan Entry-Exit Inspection and Quarantine Bureau, Haikou, Hainan 570311, China
  • Received Date: 2018-09-25
  • Rev Recd Date: 2018-09-29

Abstract: A gene of response regulator,OnRR9 gene（ GenBank accession number: MH758789）,was cloned from the petals of Oncidium Gower Ramsey flowers with RACE technology. The OnRR9 gene is 627 bp in length and encodes a protein of 208 amino acids in size. The secondary structure analysis of the protein showed that OnRR9 protein is a hydrophilic secreted protein localized in the nucleus. The homologous sequence alignment revealed that the OnRR9 gene belongs to the YesN/AraC family and contains a conserved REC domain in the amino acid sequence,which may be involved in the two-component signal transduction pathway. The construction of the phylogenetic tree demonstrated that the OnRR9 protein is closely related to the type A ARRs in Arabidopsis thaliana,suggesting that the OnRR9 may be involved in the response of cytokinin signaling. Real-time fluorescence quantitative analysis indicated that the OnRR9 gene had the maximum expression in the full blossom flower but was low expressed before and after full blossom,suggesting that this gene might play a role in the iniitation of petal senescence of Oncidium Gower Ramsey flowers.

YAN Bingyu, GONG Xiaoxiao, TAN Yurong, WANG Peng, LI Shuangjiang, PAN Yingwen, LIU Jinping. Cloning and Expression Analysis of OnRR9 Gene in Oncidium[J]. Journal of Tropical Biology, 2018, 9(4): 393-400. doi: 10.15886/j.cnki.rdswxb.2018.04.005
Citation: YAN Bingyu, GONG Xiaoxiao, TAN Yurong, WANG Peng, LI Shuangjiang, PAN Yingwen, LIU Jinping. Cloning and Expression Analysis of OnRR9 Gene in Oncidium[J]. Journal of Tropical Biology, 2018, 9(4): 393-400. doi: 10.15886/j.cnki.rdswxb.2018.04.005
shu

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