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Volume 9 Issue 3
Jun.  2018
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Article Navigation >  Journal of Tropical Biology  > 2018  >  9(3): 281-286

HU Shikang, WANG Bo, QIN Haipeng, LIAO Xuzheng, SUN Chengbo, LIU Yongsheng, QU Peng. Effect of Microbial Antimicrobial Peptides on Disease Resistance and Immune Indices of Litopenaeus vannamei[J]. Journal of Tropical Biology, 2018, 9(3): 281-286. doi: 10.15886/j.cnki.rdswxb.2018.03.003
Citation: HU Shikang, WANG Bo, QIN Haipeng, LIAO Xuzheng, SUN Chengbo, LIU Yongsheng, QU Peng. Effect of Microbial Antimicrobial Peptides on Disease Resistance and Immune Indices of Litopenaeus vannamei[J]. Journal of Tropical Biology, 2018, 9(3): 281-286. doi: 10.15886/j.cnki.rdswxb.2018.03.003
shu

Effect of Microbial Antimicrobial Peptides on Disease Resistance and Immune Indices of Litopenaeus vannamei

doi: 10.15886/j.cnki.rdswxb.2018.03.003

  • 1. Laboratory of Crustacean Genetics, Breeding and Reproduction, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, Guangdong, China;

  • 2. Engineering and Technology Research Center of Tropical Invertebrate Animal Breeding, Guangdong University, Zhanjiang 524025, China;

  • 3. Yantai Fisheries Research Institute, Yantai City, Shandong 264003;

  • 4. Shandong Shenhai Biotechnology Co, Ltd., Yantai 265500, Shandong, China

  • Received Date: 2018-07-01
  • Rev Recd Date: 2018-08-27
  • Litopenaeus vannamei weighing（ 0. 163 ± 0. 07） g were cultured with feed added with 0. 2%,0. 5% and 1. 0% of microbial antimicrobial peptide S200（ AMP S200）. After 12 weeks,the L. vannamei were inoculated with white spot syndrome virus（ WSSRV）,Vibrio harveyi and Vibrio parahaemolyticus,respectively and cultured for 16 weeks. The results showed that the addition of 0. 2% and 0. 5% of the AMP S200 in the feed significantly increased the survival rate and the activity of lysozyme and superoxide dismutase of L. vannamei challenged. When the AMP S200 was added at a rate of 0. 2% into the feed,the cumulative mortality rate of L.vannamei challenged with V. parahaemolyticus was 10. 0%,the lowest. When the feed was added with AMP S200 at a rate of 0. 5%,the L. vannamei showed cumulative mortality rates of 13. 0% and 20. 0%,respectively when challenged with V. harveyi and WSSV,and 40. 0% when challenged with V. parahaemolyticus. The activities of lysozyme and superoxide dismutase were 44. 35 U · mg-1 prot and 60. 8 U · mg-1 prot,respectively,which were significantly higher than those of the control group（ P ＜ 0. 05）. When the feed was added with AMP S200 at a rate of 1. 0%,the cumulative mortality rates of L. vannamei challenged with WSSV,V. harveyi,and V. parahaemolyticus were 53. 33%,20. 0% and 40. 0%,respectively. The activities of lysozyme and superoxide dismutase were 9. 8 U·mg-1 prot and 50. 17 U·mg-1 prot,respectively. Compared with the group treated with the feed added with AMP S200 at a rate of 0. 5%,all the figures were low in the group treated with the AMP S200 at a rate of 1. 0% except for the cumulative mortality rate of L. vannamei challenged with V. parahaemolyticus（ 40. 0%）. The higher rate of AMP S200 showed a certain negative effect on the virus resistance of L.vannamei. Compared with the control groups,the L. vannamei treated with AMP S200 at a higher ratewas not significantly different in lysozyme activity（ P ＞ 0. 05）. The cumulative mortality rates of L. vannamei challenged with V. parahaemolyticus and WSSV were significantly higher than those of the control groups（ P ＜ 0. 05）. These results showed that adding of an appropriate amount of AMP S200 in feed can increase the survival rate and the activities of superoxide dismutase and lysozyme of L. vannamei challenged with WSSV,V. harveyi,and V. parahaemolyticus,and improve the immunity of L. vannamei,contributing to the success of L. vannamei culture.
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Effect of Microbial Antimicrobial Peptides on Disease Resistance and Immune Indices of Litopenaeus vannamei

doi: 10.15886/j.cnki.rdswxb.2018.03.003
  • 1. Laboratory of Crustacean Genetics, Breeding and Reproduction, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, Guangdong, China;
  • 2. Engineering and Technology Research Center of Tropical Invertebrate Animal Breeding, Guangdong University, Zhanjiang 524025, China;
  • 3. Yantai Fisheries Research Institute, Yantai City, Shandong 264003;
  • 4. Shandong Shenhai Biotechnology Co, Ltd., Yantai 265500, Shandong, China
  • Received Date: 2018-07-01
  • Rev Recd Date: 2018-08-27

Abstract: Litopenaeus vannamei weighing（ 0. 163 ± 0. 07） g were cultured with feed added with 0. 2%,0. 5% and 1. 0% of microbial antimicrobial peptide S200（ AMP S200）. After 12 weeks,the L. vannamei were inoculated with white spot syndrome virus（ WSSRV）,Vibrio harveyi and Vibrio parahaemolyticus,respectively and cultured for 16 weeks. The results showed that the addition of 0. 2% and 0. 5% of the AMP S200 in the feed significantly increased the survival rate and the activity of lysozyme and superoxide dismutase of L. vannamei challenged. When the AMP S200 was added at a rate of 0. 2% into the feed,the cumulative mortality rate of L.vannamei challenged with V. parahaemolyticus was 10. 0%,the lowest. When the feed was added with AMP S200 at a rate of 0. 5%,the L. vannamei showed cumulative mortality rates of 13. 0% and 20. 0%,respectively when challenged with V. harveyi and WSSV,and 40. 0% when challenged with V. parahaemolyticus. The activities of lysozyme and superoxide dismutase were 44. 35 U · mg-1 prot and 60. 8 U · mg-1 prot,respectively,which were significantly higher than those of the control group（ P ＜ 0. 05）. When the feed was added with AMP S200 at a rate of 1. 0%,the cumulative mortality rates of L. vannamei challenged with WSSV,V. harveyi,and V. parahaemolyticus were 53. 33%,20. 0% and 40. 0%,respectively. The activities of lysozyme and superoxide dismutase were 9. 8 U·mg-1 prot and 50. 17 U·mg-1 prot,respectively. Compared with the group treated with the feed added with AMP S200 at a rate of 0. 5%,all the figures were low in the group treated with the AMP S200 at a rate of 1. 0% except for the cumulative mortality rate of L. vannamei challenged with V. parahaemolyticus（ 40. 0%）. The higher rate of AMP S200 showed a certain negative effect on the virus resistance of L.vannamei. Compared with the control groups,the L. vannamei treated with AMP S200 at a higher ratewas not significantly different in lysozyme activity（ P ＞ 0. 05）. The cumulative mortality rates of L. vannamei challenged with V. parahaemolyticus and WSSV were significantly higher than those of the control groups（ P ＜ 0. 05）. These results showed that adding of an appropriate amount of AMP S200 in feed can increase the survival rate and the activities of superoxide dismutase and lysozyme of L. vannamei challenged with WSSV,V. harveyi,and V. parahaemolyticus,and improve the immunity of L. vannamei,contributing to the success of L. vannamei culture.

HU Shikang, WANG Bo, QIN Haipeng, LIAO Xuzheng, SUN Chengbo, LIU Yongsheng, QU Peng. Effect of Microbial Antimicrobial Peptides on Disease Resistance and Immune Indices of Litopenaeus vannamei[J]. Journal of Tropical Biology, 2018, 9(3): 281-286. doi: 10.15886/j.cnki.rdswxb.2018.03.003
Citation: HU Shikang, WANG Bo, QIN Haipeng, LIAO Xuzheng, SUN Chengbo, LIU Yongsheng, QU Peng. Effect of Microbial Antimicrobial Peptides on Disease Resistance and Immune Indices of Litopenaeus vannamei[J]. Journal of Tropical Biology, 2018, 9(3): 281-286. doi: 10.15886/j.cnki.rdswxb.2018.03.003
shu

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