Effects of Pleocidin and Spinetoram on the Development of Zebrafish Embryos

Key words: 
• pleocidin /
• spinetoram /
• zebrafish /
• embryonic development /
• toxicity

Abstract: Zebrafish（ Danino rerio AB） was treated with pleocidin and spinetoram at different concentrations(120,151,190,240,302,380,480 mg·L^-1 for pleocidin,and 0. 6,1. 8,5. 45,16. 4 and 49. 4 mg·L^-1 for spinetoram) to observe the effect of the two chemicals on the embryo development of zebrafish. The test followed strictly the OECD guidelines for testing of fish embryo acute toxicity（ FET）. The results showed that the median lethal concentration（ LC₅₀） for zebrafish embryos exposed to pleocidin after 96 h of fertilization was 242. 27 mg·L^-1,and 9. 713 mg·L^-1 when exposed to spinetoram. Pleocidin and spinetoram had no dosage-response relationships in the frequency of spontaneous movement in 1 min after 24 h of fertilization,the frequency of heart beat in 20 s after 48 h of fertilization,and the body length after 96 h of pollination. Pleocidin and spinetoram showed a dosage-response relationship in the frequency of spinal curvature after 72 h of fertilization. These two chemicals were all low in toxicity,and in high concentrations,pleocidin is more friendly to Zebrafish than spinetoram.