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Volume 9 Issue 2
Apr.  2018
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Article Navigation >  Journal of Tropical Biology  > 2018  >  9(2): 147-155

RUAN Sunlan, ZHAO Hongwei, ZHANG Yu, ZHANG Tengyun, ZHANG Mingshan, JIN Xiaotuo, LI Ping, DIAO Xiaoping. Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China[J]. Journal of Tropical Biology, 2018, 9(2): 147-155. doi: 10.15886/j.cnki.rdswxb.2018.02.004
Citation: RUAN Sunlan, ZHAO Hongwei, ZHANG Yu, ZHANG Tengyun, ZHANG Mingshan, JIN Xiaotuo, LI Ping, DIAO Xiaoping. Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China[J]. Journal of Tropical Biology, 2018, 9(2): 147-155. doi: 10.15886/j.cnki.rdswxb.2018.02.004
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Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China

doi: 10.15886/j.cnki.rdswxb.2018.02.004

  • 1. State Key Laboratory of Marine Resource Utilization of South China Sea, Hainan University, Haikou, Hainan 570228, China;

  • 2. Haikou Key Laboratory of Environmental Toxicology, Hainan University, Haikou, Hainan 570228, China;

  • 3. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China;

  • 4. Hainan Research Academy of Environmental Science & Hainan Environmental Monitoring Center, Haikou, Hainan 570228, China

  • Received Date: 2018-01-04
  • Rev Recd Date: 2018-02-23
  • The long-term abuse of antibiotics has led to the rapid growth of drug-resistant bacteria in the environment. The distribution and mechanism of antibiotics resistance genes（ ARGs） have attracted public attention.ARGs,as an emerging contaminant,not only have a serious impact on the environment,but also pose a potential threat to the health of organisms and human beings. The surface sediment of the mangrove area in Dongzhai Harbor,Haikou,Hainan,China was collected in the mangroves,near the mangroves and the mud flats in August,2017 to determine the abundance of ARGs by using real-time quantitative PCR in a view to investigate the pollution of chloramphenicol resistance genes（ CRGs） in this area. The distribution of CRGs in the sediment samples in the mangroves,near the mangroves and in the mud flats was analyzed and compared,and the correlation between the CRGs and the environmental factors were analyzed. The results showed that this area was seriously polluted by CRGs,with an absolute abundance of CRGs being 108 copies · g-1. CRGs with different resistance mechanisms had different compositional ratios in the environmental samples,of which the cml gene was always the highest in the compositional ratios of all the samples,indicating that the resistant mechanism of CRGs is based on active efflux of antibiotics. The sediment samples in the mangroves showed higher absolute abundance of CRGs than those near the mangroves or in the mud flats. Correlation analysis between the CRGs and the environmental factors showed that NO2--N was highly significantly correlated with the CRGs.
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Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China

doi: 10.15886/j.cnki.rdswxb.2018.02.004
  • 1. State Key Laboratory of Marine Resource Utilization of South China Sea, Hainan University, Haikou, Hainan 570228, China;
  • 2. Haikou Key Laboratory of Environmental Toxicology, Hainan University, Haikou, Hainan 570228, China;
  • 3. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China;
  • 4. Hainan Research Academy of Environmental Science & Hainan Environmental Monitoring Center, Haikou, Hainan 570228, China
  • Received Date: 2018-01-04
  • Rev Recd Date: 2018-02-23

Abstract: The long-term abuse of antibiotics has led to the rapid growth of drug-resistant bacteria in the environment. The distribution and mechanism of antibiotics resistance genes（ ARGs） have attracted public attention.ARGs,as an emerging contaminant,not only have a serious impact on the environment,but also pose a potential threat to the health of organisms and human beings. The surface sediment of the mangrove area in Dongzhai Harbor,Haikou,Hainan,China was collected in the mangroves,near the mangroves and the mud flats in August,2017 to determine the abundance of ARGs by using real-time quantitative PCR in a view to investigate the pollution of chloramphenicol resistance genes（ CRGs） in this area. The distribution of CRGs in the sediment samples in the mangroves,near the mangroves and in the mud flats was analyzed and compared,and the correlation between the CRGs and the environmental factors were analyzed. The results showed that this area was seriously polluted by CRGs,with an absolute abundance of CRGs being 108 copies · g-1. CRGs with different resistance mechanisms had different compositional ratios in the environmental samples,of which the cml gene was always the highest in the compositional ratios of all the samples,indicating that the resistant mechanism of CRGs is based on active efflux of antibiotics. The sediment samples in the mangroves showed higher absolute abundance of CRGs than those near the mangroves or in the mud flats. Correlation analysis between the CRGs and the environmental factors showed that NO2--N was highly significantly correlated with the CRGs.

RUAN Sunlan, ZHAO Hongwei, ZHANG Yu, ZHANG Tengyun, ZHANG Mingshan, JIN Xiaotuo, LI Ping, DIAO Xiaoping. Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China[J]. Journal of Tropical Biology, 2018, 9(2): 147-155. doi: 10.15886/j.cnki.rdswxb.2018.02.004
Citation: RUAN Sunlan, ZHAO Hongwei, ZHANG Yu, ZHANG Tengyun, ZHANG Mingshan, JIN Xiaotuo, LI Ping, DIAO Xiaoping. Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China[J]. Journal of Tropical Biology, 2018, 9(2): 147-155. doi: 10.15886/j.cnki.rdswxb.2018.02.004
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