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Volume 9 Issue 2
Apr.  2018
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Article Navigation >  Journal of Tropical Biology  > 2018  >  9(2): 142-146

ZHANG Jingya, LIU Yupeng, WU Chao, DENG Yiqin, YIN Haoxian, ZHAO Zhe. Isolation and Enzyme Activity Assay of An Agarase-producing Vibrio sp. Strain[J]. Journal of Tropical Biology, 2018, 9(2): 142-146. doi: 10.15886/j.cnki.rdswxb.2018.02.003
Citation: ZHANG Jingya, LIU Yupeng, WU Chao, DENG Yiqin, YIN Haoxian, ZHAO Zhe. Isolation and Enzyme Activity Assay of An Agarase-producing Vibrio sp. Strain[J]. Journal of Tropical Biology, 2018, 9(2): 142-146. doi: 10.15886/j.cnki.rdswxb.2018.02.003
shu

Isolation and Enzyme Activity Assay of An Agarase-producing Vibrio sp. Strain

doi: 10.15886/j.cnki.rdswxb.2018.02.003

  • 1. Hefei Tenth Middle School, Hefei, Anhui, 230011, China;

  • 2. College of Oceanography, Hohai University, Nanjing 210098, China;

  • 3. Key Laboratory of Applied Marine Biology of Guangdong Province/South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;

  • 4. College of Life Sciences, Guangzhou University, Guangzhou 510006, China

  • Received Date: 2017-12-31
  • Rev Recd Date: 2018-04-09
  • Agarases are the enzymes which catalyze the hydrolysis of agar. They have wide applications in food industry, cosmetics, and medical fields since they produce oligosaccharides with remarkable enzyme activities. A Vibrio sp. strain, named HN897, was isolated from seawater samples in the South China coastal area using TCBS agar plate. This strain was identified as an agarase-producing bacterium by observation of clear zones around the colonies using agar as the sole carbon source. Phylogenetic analysis based on 16 S r RNA gene shows that the agar-degrading bacterium HN897 belongs to the member of the genus Vibrio and is close to Vibrio astriarenae.The agarase activity of the strain was determined by using reducing dinitrosalicylic acid method（DNS method）, and the agarase activities of the crude enzymes in the supernatant of HN897 culture of the TSB and LB mediums were0.366 U·m L-1 and 0.413 U·m L-1, respectively, indicating that this strain has potential applications in the production of agaro-oligosaccharide.
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Isolation and Enzyme Activity Assay of An Agarase-producing Vibrio sp. Strain

doi: 10.15886/j.cnki.rdswxb.2018.02.003
  • 1. Hefei Tenth Middle School, Hefei, Anhui, 230011, China;
  • 2. College of Oceanography, Hohai University, Nanjing 210098, China;
  • 3. Key Laboratory of Applied Marine Biology of Guangdong Province/South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;
  • 4. College of Life Sciences, Guangzhou University, Guangzhou 510006, China
  • Received Date: 2017-12-31
  • Rev Recd Date: 2018-04-09

Abstract: Agarases are the enzymes which catalyze the hydrolysis of agar. They have wide applications in food industry, cosmetics, and medical fields since they produce oligosaccharides with remarkable enzyme activities. A Vibrio sp. strain, named HN897, was isolated from seawater samples in the South China coastal area using TCBS agar plate. This strain was identified as an agarase-producing bacterium by observation of clear zones around the colonies using agar as the sole carbon source. Phylogenetic analysis based on 16 S r RNA gene shows that the agar-degrading bacterium HN897 belongs to the member of the genus Vibrio and is close to Vibrio astriarenae.The agarase activity of the strain was determined by using reducing dinitrosalicylic acid method（DNS method）, and the agarase activities of the crude enzymes in the supernatant of HN897 culture of the TSB and LB mediums were0.366 U·m L-1 and 0.413 U·m L-1, respectively, indicating that this strain has potential applications in the production of agaro-oligosaccharide.

ZHANG Jingya, LIU Yupeng, WU Chao, DENG Yiqin, YIN Haoxian, ZHAO Zhe. Isolation and Enzyme Activity Assay of An Agarase-producing Vibrio sp. Strain[J]. Journal of Tropical Biology, 2018, 9(2): 142-146. doi: 10.15886/j.cnki.rdswxb.2018.02.003
Citation: ZHANG Jingya, LIU Yupeng, WU Chao, DENG Yiqin, YIN Haoxian, ZHAO Zhe. Isolation and Enzyme Activity Assay of An Agarase-producing Vibrio sp. Strain[J]. Journal of Tropical Biology, 2018, 9(2): 142-146. doi: 10.15886/j.cnki.rdswxb.2018.02.003
shu

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