Effects of Different Hormone Combinations on Callus Inducing of Shoot Explant from Bitter Leaf（Vernonia amygdallina Del）

Key words: 
• Vernonia amygdalina Del /
• stem explants /
• different hormones /
• callus /
• inducing rate

Abstract: Young shoots of the stem of Bitter Leaf（ Vernonia amygdalina Del） were used as explants and cultured in the 1/2 MS with supplements of combinations of three plant hormones,ie. NAA,6-BA and 2,4-D,at different concentrations to screen the optimum hormone combinations for tissue culture. An orthogonal experimental design was arranged for the 3 plant hormones at 5 levels of concentration,and 25 optimal treatments were selected with 3 replicates each treatment for the experiment. The 1/2 MS with the combinations of the plant hormones was added with 50 g·L^-1 sucrose and 1. 0 g·L^-1 Hyponex in each petri dish to induce calli from the explants. The results showed that in the combinations of NAA and 6-BA the callus inducing rate was the highest,upto 97. 8%,in the medium supplemented with 2 mg·L^-1 NAA and 0. 5 mg·L^-16-BA,and that in the combinations of NAA and 2,4-D the callus inducing was the highest in the medium supplemented with 1. 5 mg·L^-1 NAA and 1. 0 mg·L^-12,4-D. No calli were induced in the medium supplemented only with NAA.