Cloning and Expression Analysis of MeHDS09 Gene Related to Abiotic Stress Signals in Cassava

Key words: 
• MeHDS09 /
• cassava /
• abiotic stress /
• bioinformatic analysis /
• expression profiles

Abstract: The gene MeHDS09 was cloned from cassava to analyze its structure and functions in tissue cultured plants under different abiotic stresses. MeHDS09 was isolated from cassava plant using RT-PCR and had 2 151 bp open reading frame encoding 716 amino acids according to the transcriptome data and genome-wide public sequences on cassava. The MeHDS09 encoded protein was 81. 2 k D in molecular weight,and did not contain the signal peptide site. Subcellular localization prediction showed that MeHDS09 contained mitochondrial targeting peptide and was mainly localized in mitochondrion. Comparison of sequences similarity with reported genes sequences in Genbank showed that MeHDS09 might have higher homology with Protein 2 in soybean,which was related to enhancement of plant disease resistance. The promoter sequence analysis showed that the upstream promoter sequence of the MeHDS09 contained not only promoter core elements but also hormone responsive elements in response to disease resistance and hormones. This indicated that the expression of MeHDS09 might be influenced by the external environment factors. Further analysis with qRP-PCR showed that the MeHDS09 was not sensitive in response to GA/IAA,and that the cold,ABA and JA treatments induced significant expression of MeHDS09 in cassava,while the hot and SA treatments inhibited obviously expression of the MeHDS09. The results revealed that MeHDS09 might play a role in resistance to abiotic stresses in cassava,such as low temperature.