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Volume 8 Issue 4
Dec.  2017
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Article Navigation >  Journal of Tropical Biology  > 2017  >  8(4): 377-382

YI Xuejie, ZHANG Hua, HE Maoxian. Biomineralization Function of ChSy-1 in Pearl Oyster Pinctada fucata[J]. Journal of Tropical Biology, 2017, 8(4): 377-382. doi: 10.15886/j.cnki.rdswxb.2017.04.001
Citation: YI Xuejie, ZHANG Hua, HE Maoxian. Biomineralization Function of ChSy-1 in Pearl Oyster Pinctada fucata[J]. Journal of Tropical Biology, 2017, 8(4): 377-382. doi: 10.15886/j.cnki.rdswxb.2017.04.001
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Biomineralization Function of ChSy-1 in Pearl Oyster Pinctada fucata

doi: 10.15886/j.cnki.rdswxb.2017.04.001

  • 1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, CAS, Guangzhou, Guangdong 510301, China;

  • 2. Guangdong Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, China;

  • 3. University of Chinese Academy of Sciences, Beijing 100049, China

  • Received Date: 2017-01-17
  • Rev Recd Date: 2017-03-07
  • In order to investigate the mechanism of chondroitin sulfate synthase 1（ ChSy-1） participating in biomineralization in the pearl oyster Pinctada fucata,we performed tissue distribution experiment,shell notching experiment and RNA interference test. Results showed that ChSy-1 gene was expressed in different parts of the mantle of P. fucata,,highly in the edge of the mantle. At the different developmental stages,the expression of ChSy-1 was higher at the polar body phase,2-cell stage and 32-cell stage. The results of shell notching experiment showed that the expression of ChSy-1 gene increased significantly after 48 and 72 hours,and that the expression level of ChSy-1 also increased significantly after knockout of BMP2 gene,which is consistent with the mechanism by which ChSy-1 plays a negative regulatory role in the BMP signaling pathway. This study shows that ChSy-1 plays an important role in the biomineralization of P. fucata,which provides important information for the characterization of ChSy-1 in the biomineralization of P. fucata.
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Biomineralization Function of ChSy-1 in Pearl Oyster Pinctada fucata

doi: 10.15886/j.cnki.rdswxb.2017.04.001
  • 1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, CAS, Guangzhou, Guangdong 510301, China;
  • 2. Guangdong Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, China;
  • 3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received Date: 2017-01-17
  • Rev Recd Date: 2017-03-07

Abstract: In order to investigate the mechanism of chondroitin sulfate synthase 1（ ChSy-1） participating in biomineralization in the pearl oyster Pinctada fucata,we performed tissue distribution experiment,shell notching experiment and RNA interference test. Results showed that ChSy-1 gene was expressed in different parts of the mantle of P. fucata,,highly in the edge of the mantle. At the different developmental stages,the expression of ChSy-1 was higher at the polar body phase,2-cell stage and 32-cell stage. The results of shell notching experiment showed that the expression of ChSy-1 gene increased significantly after 48 and 72 hours,and that the expression level of ChSy-1 also increased significantly after knockout of BMP2 gene,which is consistent with the mechanism by which ChSy-1 plays a negative regulatory role in the BMP signaling pathway. This study shows that ChSy-1 plays an important role in the biomineralization of P. fucata,which provides important information for the characterization of ChSy-1 in the biomineralization of P. fucata.

YI Xuejie, ZHANG Hua, HE Maoxian. Biomineralization Function of ChSy-1 in Pearl Oyster Pinctada fucata[J]. Journal of Tropical Biology, 2017, 8(4): 377-382. doi: 10.15886/j.cnki.rdswxb.2017.04.001
Citation: YI Xuejie, ZHANG Hua, HE Maoxian. Biomineralization Function of ChSy-1 in Pearl Oyster Pinctada fucata[J]. Journal of Tropical Biology, 2017, 8(4): 377-382. doi: 10.15886/j.cnki.rdswxb.2017.04.001
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