The Development of Airway Inflammation Murine Model Induced by Recombinant Blomia tropicalis Group 21 Allergen

Key words: 
• Blomia tropicalis /
• recombinant Blo t 21 /
• airway inflammation

Abstract: An attempt was made to develop airway inflammation murine model induced by the recombinant Blo t21 allergen.The Blo t 21 gene sequences were obtained from NCBI Gen Bank,then optimized,synthesized and inserted into the prokaryotic expression vector PQE80 L.The recombinant Blo t 21 was expressed in E.coli bacteria,and purified by NI-NTA agarose.Twelve BALB/c mice were divided randomly into control group（PBS group,n=6） and rBlo t 21-induced group（rBlo t 21 group,n=6）.After sensitization by intraperitoneal injection（i.p） and challenged by intratracheal instillation of rBlo t 21,the total serum IgE levels were measured by ELISA;the lungs were fixed and stained with haematoxylin and eosin（H＆E）.The recombinant Blo t 21 allergen was expressed successfully in E.coli and purified by Ni-NTA affinity chromatography.Furthermore,the airway inflammation murine model were induced by the recombinant Blo t 21 allergen.Compared with the control group（PBS group）,the total serum IgE levels rose obviously in the rBlo t 21 group（p＜0.005）.Mice in the rBlo t 21 group showed inflammatory cell infiltration in airway by haematoxylin and eosin（H＆E）.The airway inflammation murine model was induced successfully by the recombinant Blo t 21 allergen.