Chromosome Physical Location of 11 Members of Alkaline or Neutral Invertase Gene Family in Cassava

Key words: 
• Cassava /
• N/A-lnv gene family /
• FISH /
• in situ PCR /
• physical localization

Abstract: Alkaline or neutral invertase（N/A-lnv） is a key enzyme for starch synthesis process in cassava.Chromosome specimens were prepared from the bronze-coloured tender leaves of cassava variety SC6,and 11 members of the cassava neutral or alkaline invertase gene family were physically localized by fluorescence in situ hybridization（FISH） and in situ PCR.The results showed that genes MeNINV5,MeNINV9 and MeNINV10 were located on the chromosome 4.MeNINV9 and MeNINV10 had percentage distances of 68.52 and 95.35 from the signal locus to the centromere on the short arm,respectively,and MeNINV5 had a percentage distance of 22.71 from the signal locus to the centromere on the long arm.MeNINV4 and nINV1 genes were located on the long arm of chromosome 6,and their percentage distances from the signal locus to the centromere are 43.16 and 80.71,respectively;MeNINV6 and MeNINV7 genes were located on the long arms of chromosome 7 and 17,respectively,and their percentage distances from the signal locus to the centromere are 15.38 and 57.97,respectively;MeNINV1,MeNINV2,MeNINV3 and MeNINV8 genes were located on the short arms of chromosome 11,9,5 and 16,respectively,and their percentage distances from the signal locus to the centromere were 40.10,51.88,91.75 and 76.33,respectively.The linkage relationship was discussed between the N/A-lnv gene family and other genes located on cassava chromosomes.