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Volume 7 Issue 2
May  2016
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Article Navigation >  Journal of Tropical Biology  > 2016  >  7(2): 259-264

ZHU Jinfeng, SUN Huizhong, WANG Xiaodong, LI Guangliang, SUN Minghui, CHEN Chong, CHEN Qilong. Isolation and Identification of Phosphate Accumulating Strain YYF-3 and its Growth Characteristics[J]. Journal of Tropical Biology, 2016, 7(2): 259-264. doi: 10.15886/j.cnki.rdswxb.2016.02.020
Citation: ZHU Jinfeng, SUN Huizhong, WANG Xiaodong, LI Guangliang, SUN Minghui, CHEN Chong, CHEN Qilong. Isolation and Identification of Phosphate Accumulating Strain YYF-3 and its Growth Characteristics[J]. Journal of Tropical Biology, 2016, 7(2): 259-264. doi: 10.15886/j.cnki.rdswxb.2016.02.020
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Isolation and Identification of Phosphate Accumulating Strain YYF-3 and its Growth Characteristics

doi: 10.15886/j.cnki.rdswxb.2016.02.020

  • 1. Henan Tobacco Company Luohe Branch, Luohe, Henan 462000, China;

  • 2. College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471003, China

  • Received Date: 2015-07-26
  • A bacterial strain with high phosphate solubilizing activity was isolated from rhizosphere soil of tobacco（ Nicotiana tabacum L.） by enrichment culture and was screen cultured for preliminary identification,and it was coded strain YYF-3. After 48 hr of fermentation the strain YYF-3 was found to contain 221. 7 mg·L-1of free phosphorus in the culture supernatant,which was further proved that this strain had higher phosphate solubilizing ability. Morphological observation,biochemical detection,and 16 S r DNA sequence analysis showed the strain YYFG3 was grouped to the genus Acinetobacter,temporarily named Acinetobacter sp. The growth culture showed that the strains YYF-3 grew best under the temperature of 28- 34 ℃ with the optimum p H ranging from 6. 0 to 6. 5,and gave a sensitive response to Na Cl. The strain YYF-3 can be used as good material for mutation breeding and whole-genome based breeding for phosphate solubilizing bacterial microbes,and can also be used as a standby strain for preparation of tobacco special biofertilizer.
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Isolation and Identification of Phosphate Accumulating Strain YYF-3 and its Growth Characteristics

doi: 10.15886/j.cnki.rdswxb.2016.02.020
  • 1. Henan Tobacco Company Luohe Branch, Luohe, Henan 462000, China;
  • 2. College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471003, China
  • Received Date: 2015-07-26

Abstract: A bacterial strain with high phosphate solubilizing activity was isolated from rhizosphere soil of tobacco（ Nicotiana tabacum L.） by enrichment culture and was screen cultured for preliminary identification,and it was coded strain YYF-3. After 48 hr of fermentation the strain YYF-3 was found to contain 221. 7 mg·L-1of free phosphorus in the culture supernatant,which was further proved that this strain had higher phosphate solubilizing ability. Morphological observation,biochemical detection,and 16 S r DNA sequence analysis showed the strain YYFG3 was grouped to the genus Acinetobacter,temporarily named Acinetobacter sp. The growth culture showed that the strains YYF-3 grew best under the temperature of 28- 34 ℃ with the optimum p H ranging from 6. 0 to 6. 5,and gave a sensitive response to Na Cl. The strain YYF-3 can be used as good material for mutation breeding and whole-genome based breeding for phosphate solubilizing bacterial microbes,and can also be used as a standby strain for preparation of tobacco special biofertilizer.

ZHU Jinfeng, SUN Huizhong, WANG Xiaodong, LI Guangliang, SUN Minghui, CHEN Chong, CHEN Qilong. Isolation and Identification of Phosphate Accumulating Strain YYF-3 and its Growth Characteristics[J]. Journal of Tropical Biology, 2016, 7(2): 259-264. doi: 10.15886/j.cnki.rdswxb.2016.02.020
Citation: ZHU Jinfeng, SUN Huizhong, WANG Xiaodong, LI Guangliang, SUN Minghui, CHEN Chong, CHEN Qilong. Isolation and Identification of Phosphate Accumulating Strain YYF-3 and its Growth Characteristics[J]. Journal of Tropical Biology, 2016, 7(2): 259-264. doi: 10.15886/j.cnki.rdswxb.2016.02.020
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