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Volume 7 Issue 1
Jan.  2016
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Article Navigation >  Journal of Tropical Biology  > 2016  >  7(1): 124-127

XU Xiandong, ZHANG Guoqing, XIE Zhenyu, WANG Shifeng, ZHOU Yongcan. Improvement of Filter Paper Pack for Bacteria Sample Preparation for Scanning Electron Microscope Observation[J]. Journal of Tropical Biology, 2016, 7(1): 124-127. doi: 10.15886/j.cnki.rdswxb.2016.01.021
Citation: XU Xiandong, ZHANG Guoqing, XIE Zhenyu, WANG Shifeng, ZHOU Yongcan. Improvement of Filter Paper Pack for Bacteria Sample Preparation for Scanning Electron Microscope Observation[J]. Journal of Tropical Biology, 2016, 7(1): 124-127. doi: 10.15886/j.cnki.rdswxb.2016.01.021
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Improvement of Filter Paper Pack for Bacteria Sample Preparation for Scanning Electron Microscope Observation

doi: 10.15886/j.cnki.rdswxb.2016.01.021

  • 1. Ocean College, Hainan University, Haikou, Hainan 570228;

  • 2. Hainan Key Laboratory for Tropical Hydrobiology and Biotechnology, Haikou, Hainan 570228;

  • 3. Jiangxi Fisheries Research Institute, Nanchang, Jiangxi 330039, China

  • Received Date: 2015-09-10
  • The filter paper pack method is generally used to prepare SEM sample for observation of bacteria and other mono-cell creatures,and it saves time and samples as compared to the traditional method,However,with this method some samples can’t be distinguished by naked eye in the filter paper after freeze drying. Moreover,the electron micrograph obtained by this method has interference of filter paper background,and it is thus difficult to observe the subtle structure of bacteria on the micrograph. In this context,the filter paper pack was improved by adding a layer of tinfoil onto the filter paper pack as lining,and the samples were dropped on the tinfoil before ethanol dehydration operations. With the improved method the distribution of sample on the foil can be easily distinguished by naked eye without interference of filter paper background,and the subtle structure of bacteria surface can be clearly observed.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Improvement of Filter Paper Pack for Bacteria Sample Preparation for Scanning Electron Microscope Observation

doi: 10.15886/j.cnki.rdswxb.2016.01.021
  • 1. Ocean College, Hainan University, Haikou, Hainan 570228;
  • 2. Hainan Key Laboratory for Tropical Hydrobiology and Biotechnology, Haikou, Hainan 570228;
  • 3. Jiangxi Fisheries Research Institute, Nanchang, Jiangxi 330039, China
  • Received Date: 2015-09-10

Abstract: The filter paper pack method is generally used to prepare SEM sample for observation of bacteria and other mono-cell creatures,and it saves time and samples as compared to the traditional method,However,with this method some samples can’t be distinguished by naked eye in the filter paper after freeze drying. Moreover,the electron micrograph obtained by this method has interference of filter paper background,and it is thus difficult to observe the subtle structure of bacteria on the micrograph. In this context,the filter paper pack was improved by adding a layer of tinfoil onto the filter paper pack as lining,and the samples were dropped on the tinfoil before ethanol dehydration operations. With the improved method the distribution of sample on the foil can be easily distinguished by naked eye without interference of filter paper background,and the subtle structure of bacteria surface can be clearly observed.

XU Xiandong, ZHANG Guoqing, XIE Zhenyu, WANG Shifeng, ZHOU Yongcan. Improvement of Filter Paper Pack for Bacteria Sample Preparation for Scanning Electron Microscope Observation[J]. Journal of Tropical Biology, 2016, 7(1): 124-127. doi: 10.15886/j.cnki.rdswxb.2016.01.021
Citation: XU Xiandong, ZHANG Guoqing, XIE Zhenyu, WANG Shifeng, ZHOU Yongcan. Improvement of Filter Paper Pack for Bacteria Sample Preparation for Scanning Electron Microscope Observation[J]. Journal of Tropical Biology, 2016, 7(1): 124-127. doi: 10.15886/j.cnki.rdswxb.2016.01.021
shu

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