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Volume 7 Issue 1
Jan.  2016
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Article Navigation >  Journal of Tropical Biology  > 2016  >  7(1): 92-98

LIU Wenchao, LIU Shoubai, MEI Wenli, DAI Haofu, JIANG Changshun. Identification of Antibacterial Components from the Leaves of Mineriao Stylosanthes[J]. Journal of Tropical Biology, 2016, 7(1): 92-98. doi: 10.15886/j.cnki.rdswxb.2016.01.016
Citation: LIU Wenchao, LIU Shoubai, MEI Wenli, DAI Haofu, JIANG Changshun. Identification of Antibacterial Components from the Leaves of Mineriao Stylosanthes[J]. Journal of Tropical Biology, 2016, 7(1): 92-98. doi: 10.15886/j.cnki.rdswxb.2016.01.016
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Identification of Antibacterial Components from the Leaves of Mineriao Stylosanthes

doi: 10.15886/j.cnki.rdswxb.2016.01.016

  • 1. College of agronomy, Hainan University, Haikou, Hainan 570228, China;

  • 2. Ministry of Agriculture Key Laboratory of Biology and Genetic Resources of Tropical Crops/, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101;

  • 3. Environmental and Plant Protection Institute, CATAS, Haikou, Hainan 570101, China

  • Received Date: 2015-11-05
  • Eight compounds were isolated from the leaves of Mineriao stylosanthes（Stylosanthes guianensis‘Mineriao’） and purified by various column chromatographies on silica and sephadex LH-20 gel. Spectral analysis of NMR and MS showed that these compounds were identified based on their structures as salicylic acid（1）,medicarpin（2）,aglycone of crotonionoside F（3）,schensiand A（4）,pinoresinol（5）,3’-methoxycoumestrol（6）,stigmast-5-ene-3β,7α-diol（7） and glycerol monolinoleate（8）. Antibacterial activity assay by using the filter paper agar diffusion method showed that compound（1） had inhibitory effect on Collectotrichum gloeosporioides（Pent.） Sacc,F. oxysporum f. sp. Cuben,F. oxysporum f. sp. Vasinfectum and Verticillium dahlia Kleb,compound（2） on C. gloeosporioides（Pent.） Sacc and V. dahlia Kleb,and compound（8） on C. gloeosporioides（Pent.） Sacc.
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Identification of Antibacterial Components from the Leaves of Mineriao Stylosanthes

doi: 10.15886/j.cnki.rdswxb.2016.01.016
  • 1. College of agronomy, Hainan University, Haikou, Hainan 570228, China;
  • 2. Ministry of Agriculture Key Laboratory of Biology and Genetic Resources of Tropical Crops/, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101;
  • 3. Environmental and Plant Protection Institute, CATAS, Haikou, Hainan 570101, China
  • Received Date: 2015-11-05

Abstract: Eight compounds were isolated from the leaves of Mineriao stylosanthes（Stylosanthes guianensis‘Mineriao’） and purified by various column chromatographies on silica and sephadex LH-20 gel. Spectral analysis of NMR and MS showed that these compounds were identified based on their structures as salicylic acid（1）,medicarpin（2）,aglycone of crotonionoside F（3）,schensiand A（4）,pinoresinol（5）,3’-methoxycoumestrol（6）,stigmast-5-ene-3β,7α-diol（7） and glycerol monolinoleate（8）. Antibacterial activity assay by using the filter paper agar diffusion method showed that compound（1） had inhibitory effect on Collectotrichum gloeosporioides（Pent.） Sacc,F. oxysporum f. sp. Cuben,F. oxysporum f. sp. Vasinfectum and Verticillium dahlia Kleb,compound（2） on C. gloeosporioides（Pent.） Sacc and V. dahlia Kleb,and compound（8） on C. gloeosporioides（Pent.） Sacc.

LIU Wenchao, LIU Shoubai, MEI Wenli, DAI Haofu, JIANG Changshun. Identification of Antibacterial Components from the Leaves of Mineriao Stylosanthes[J]. Journal of Tropical Biology, 2016, 7(1): 92-98. doi: 10.15886/j.cnki.rdswxb.2016.01.016
Citation: LIU Wenchao, LIU Shoubai, MEI Wenli, DAI Haofu, JIANG Changshun. Identification of Antibacterial Components from the Leaves of Mineriao Stylosanthes[J]. Journal of Tropical Biology, 2016, 7(1): 92-98. doi: 10.15886/j.cnki.rdswxb.2016.01.016
shu

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