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Volume 6 Issue 4
Dec.  2015
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Article Navigation >  Journal of Tropical Biology  > 2015  >  6(4): 485-490

LI Shangzhen, HU Meijiao, GAO Zhaoyin, YANG Fusun. Identification of Tulasnella sp from Dendrobium sinense roots and Detection of Amino Acids in the Fermentation Broths[J]. Journal of Tropical Biology, 2015, 6(4): 485-490. doi: 10.15886/j.cnki.rdswxb.2015.04.020
Citation: LI Shangzhen, HU Meijiao, GAO Zhaoyin, YANG Fusun. Identification of Tulasnella sp from Dendrobium sinense roots and Detection of Amino Acids in the Fermentation Broths[J]. Journal of Tropical Biology, 2015, 6(4): 485-490. doi: 10.15886/j.cnki.rdswxb.2015.04.020
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Identification of Tulasnella sp from Dendrobium sinense roots and Detection of Amino Acids in the Fermentation Broths

doi: 10.15886/j.cnki.rdswxb.2015.04.020

  • 1. College of Agronomy, Hainan University, Haikou, Hainan 570228;

  • 2. Environment and Plant Protection Research Institute, Chinese Academy of Tropical Agricultural Sciences/Ministry of Agriculture Key Laboratory for Integrated Pest Management on Tropical Crops/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China

  • Received Date: 2015-04-20
  • The strain S1 was separated from the roots of Dendrobium sinense,its mycelial morphology was observed under the microscope,and the ITS-5. 8S rDNA sequences of the strains were cloned,sequenced and analyzed by using BLAST. The strain isolated was cultured in PDB and DE liquid culture media,and plant free amino acids were detected by using high performance liquid chromatography（ HPLC）. The strain S1 isolated from the roots of D. sinense was identified as Tulasnella sp. of the family Tulasnellaceae,and detected to contain 17 essential amino acids in both PDB and DE fermentation broths,of which 3 amino acids,Pro,Cys and Lys,were much higher than the other amino acids.
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Identification of Tulasnella sp from Dendrobium sinense roots and Detection of Amino Acids in the Fermentation Broths

doi: 10.15886/j.cnki.rdswxb.2015.04.020
  • 1. College of Agronomy, Hainan University, Haikou, Hainan 570228;
  • 2. Environment and Plant Protection Research Institute, Chinese Academy of Tropical Agricultural Sciences/Ministry of Agriculture Key Laboratory for Integrated Pest Management on Tropical Crops/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China
  • Received Date: 2015-04-20

Abstract: The strain S1 was separated from the roots of Dendrobium sinense,its mycelial morphology was observed under the microscope,and the ITS-5. 8S rDNA sequences of the strains were cloned,sequenced and analyzed by using BLAST. The strain isolated was cultured in PDB and DE liquid culture media,and plant free amino acids were detected by using high performance liquid chromatography（ HPLC）. The strain S1 isolated from the roots of D. sinense was identified as Tulasnella sp. of the family Tulasnellaceae,and detected to contain 17 essential amino acids in both PDB and DE fermentation broths,of which 3 amino acids,Pro,Cys and Lys,were much higher than the other amino acids.

LI Shangzhen, HU Meijiao, GAO Zhaoyin, YANG Fusun. Identification of Tulasnella sp from Dendrobium sinense roots and Detection of Amino Acids in the Fermentation Broths[J]. Journal of Tropical Biology, 2015, 6(4): 485-490. doi: 10.15886/j.cnki.rdswxb.2015.04.020
Citation: LI Shangzhen, HU Meijiao, GAO Zhaoyin, YANG Fusun. Identification of Tulasnella sp from Dendrobium sinense roots and Detection of Amino Acids in the Fermentation Broths[J]. Journal of Tropical Biology, 2015, 6(4): 485-490. doi: 10.15886/j.cnki.rdswxb.2015.04.020
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