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Volume 6 Issue 2
Apr.  2015
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Article Navigation >  Journal of Tropical Biology  > 2015  >  6(2): 168-172

ZHENG Yunke, HU Xiangyu, Song Xiqiang, WANG Jian. Optimized Extraction Method for Genomic DNA from Dendrobium Species（ Orchidaceae）[J]. Journal of Tropical Biology, 2015, 6(2): 168-172. doi: 10.15886/j.cnki.rdswxb.2015.02.011
Citation: ZHENG Yunke, HU Xiangyu, Song Xiqiang, WANG Jian. Optimized Extraction Method for Genomic DNA from Dendrobium Species（ Orchidaceae）[J]. Journal of Tropical Biology, 2015, 6(2): 168-172. doi: 10.15886/j.cnki.rdswxb.2015.02.011
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Optimized Extraction Method for Genomic DNA from Dendrobium Species（ Orchidaceae）

doi: 10.15886/j.cnki.rdswxb.2015.02.011

  • College of Horticulture and Landscape Architecture Hainan University/Ministry of Education Key Laboratory of Protection and Developmental Utilization of Tropical Crop Germplasm Resources, Haikou 570228, China

  • Received Date: 2015-04-01
  • Extracting high quality DNA is very important for molecular experiments. Dendrobium species under the family Orchidaceae contain more sucrose and polyphenol substances and,it is hence difficult to extract high quality DNA from Dendrobium species. Six methods were used to extract DNA from 7 Dendrobium species,and compared to develop an optimal extracting method for DNA. Both PEX-improved method and CTAB-improved method had positive effect on DNA extraction from Dendrobium spp. which contained more polysaccharides and polyphenols. Compared with other 5 methods,the PEX-improved method removed higher content of polysaccharides and phenolics from Dendrobium spp and met the need of molecular biology experiments. The CTAB-improved method and the SDS-improved method removed more content of polysaccharides,and produced higher yield DNA samples as compared with the CTAB method and the SDS method.
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Optimized Extraction Method for Genomic DNA from Dendrobium Species（ Orchidaceae）

doi: 10.15886/j.cnki.rdswxb.2015.02.011
  • College of Horticulture and Landscape Architecture Hainan University/Ministry of Education Key Laboratory of Protection and Developmental Utilization of Tropical Crop Germplasm Resources, Haikou 570228, China
  • Received Date: 2015-04-01

Abstract: Extracting high quality DNA is very important for molecular experiments. Dendrobium species under the family Orchidaceae contain more sucrose and polyphenol substances and,it is hence difficult to extract high quality DNA from Dendrobium species. Six methods were used to extract DNA from 7 Dendrobium species,and compared to develop an optimal extracting method for DNA. Both PEX-improved method and CTAB-improved method had positive effect on DNA extraction from Dendrobium spp. which contained more polysaccharides and polyphenols. Compared with other 5 methods,the PEX-improved method removed higher content of polysaccharides and phenolics from Dendrobium spp and met the need of molecular biology experiments. The CTAB-improved method and the SDS-improved method removed more content of polysaccharides,and produced higher yield DNA samples as compared with the CTAB method and the SDS method.

ZHENG Yunke, HU Xiangyu, Song Xiqiang, WANG Jian. Optimized Extraction Method for Genomic DNA from Dendrobium Species（ Orchidaceae）[J]. Journal of Tropical Biology, 2015, 6(2): 168-172. doi: 10.15886/j.cnki.rdswxb.2015.02.011
Citation: ZHENG Yunke, HU Xiangyu, Song Xiqiang, WANG Jian. Optimized Extraction Method for Genomic DNA from Dendrobium Species（ Orchidaceae）[J]. Journal of Tropical Biology, 2015, 6(2): 168-172. doi: 10.15886/j.cnki.rdswxb.2015.02.011
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