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Volume 6 Issue 2
Apr.  2015
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Article Navigation >  Journal of Tropical Biology  > 2015  >  6(2): 141-146

XIAO Xiaorong, SU Tonghui, LIAO Hualan, LI Xiuqiong, WANG Defeng, FU Xiumei, CHEN Yinhua, NIU Xiaolei. Identification and Analysis of OsCERK2 Transgenic Progeny of Rice[J]. Journal of Tropical Biology, 2015, 6(2): 141-146. doi: 10.15886/j.cnki.rdswxb.2015.02.006
Citation: XIAO Xiaorong, SU Tonghui, LIAO Hualan, LI Xiuqiong, WANG Defeng, FU Xiumei, CHEN Yinhua, NIU Xiaolei. Identification and Analysis of OsCERK2 Transgenic Progeny of Rice[J]. Journal of Tropical Biology, 2015, 6(2): 141-146. doi: 10.15886/j.cnki.rdswxb.2015.02.006
shu

Identification and Analysis of OsCERK2 Transgenic Progeny of Rice

doi: 10.15886/j.cnki.rdswxb.2015.02.006

  • 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou 570228, China;

  • 2. College of Agronomy, Hainan University, Haikou 570228, China

  • Received Date: 2015-04-12
  • To study the biological function of OsCERK2 gene,we constructed the OsCERK2 gene overexpression and RNA interference vectors by modifying p CAMBIA1300 and p TCK303 as framework plasmid,and then transferred the vectors respectively into the rice through Agrobacterium-mediated genetic transformation. Molecular detection produced four overexpressing homozygous lines and two RNAi homozygous lines with reduced transcriptional level. The transgenic plants of the OE lines and RNAi lines were sprayed with peptidoglycan prepared from the cell wall of both race PXO99（ Xoo- R6） of Xanthomonas oryzae pv. oryzae（ PGNXoo） and the strain JB01-25 of X. oryzae pv. oryzicola（ PGNXoc） and Chitin Mo from the cell wall of Magnaporthe oryzae,with water as control. The expression of pathogenesis related（ PR） genes were determined by using the semi-quantitative RTPCR. The results showed that the plants of overexpressing lines improved the expression of their PR genes while the plants of RNAi expressing line reduced the expression of the PR genes,as compared with the nontransgenic rice. Furthermore,the plants of overexpressing transgenic lines exhibited high tolerance to PXO99,but there were not significantly different between the RNAi plants and the wild type. These results indicated that OsCERK2 overexpressing gene may be an effective way to disease tolerance of rice.
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Identification and Analysis of OsCERK2 Transgenic Progeny of Rice

doi: 10.15886/j.cnki.rdswxb.2015.02.006
  • 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou 570228, China;
  • 2. College of Agronomy, Hainan University, Haikou 570228, China
  • Received Date: 2015-04-12

Abstract: To study the biological function of OsCERK2 gene,we constructed the OsCERK2 gene overexpression and RNA interference vectors by modifying p CAMBIA1300 and p TCK303 as framework plasmid,and then transferred the vectors respectively into the rice through Agrobacterium-mediated genetic transformation. Molecular detection produced four overexpressing homozygous lines and two RNAi homozygous lines with reduced transcriptional level. The transgenic plants of the OE lines and RNAi lines were sprayed with peptidoglycan prepared from the cell wall of both race PXO99（ Xoo- R6） of Xanthomonas oryzae pv. oryzae（ PGNXoo） and the strain JB01-25 of X. oryzae pv. oryzicola（ PGNXoc） and Chitin Mo from the cell wall of Magnaporthe oryzae,with water as control. The expression of pathogenesis related（ PR） genes were determined by using the semi-quantitative RTPCR. The results showed that the plants of overexpressing lines improved the expression of their PR genes while the plants of RNAi expressing line reduced the expression of the PR genes,as compared with the nontransgenic rice. Furthermore,the plants of overexpressing transgenic lines exhibited high tolerance to PXO99,but there were not significantly different between the RNAi plants and the wild type. These results indicated that OsCERK2 overexpressing gene may be an effective way to disease tolerance of rice.

XIAO Xiaorong, SU Tonghui, LIAO Hualan, LI Xiuqiong, WANG Defeng, FU Xiumei, CHEN Yinhua, NIU Xiaolei. Identification and Analysis of OsCERK2 Transgenic Progeny of Rice[J]. Journal of Tropical Biology, 2015, 6(2): 141-146. doi: 10.15886/j.cnki.rdswxb.2015.02.006
Citation: XIAO Xiaorong, SU Tonghui, LIAO Hualan, LI Xiuqiong, WANG Defeng, FU Xiumei, CHEN Yinhua, NIU Xiaolei. Identification and Analysis of OsCERK2 Transgenic Progeny of Rice[J]. Journal of Tropical Biology, 2015, 6(2): 141-146. doi: 10.15886/j.cnki.rdswxb.2015.02.006
shu

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