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Volume 6 Issue 2
Apr.  2015
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Article Navigation >  Journal of Tropical Biology  > 2015  >  6(2): 113-118

XIAO Hua, LIN Yunzheng, YAO Di, OUYANG Mo, HUANG Xi. Cloning and Characterization of a Zinc-finger Gene of Hevea brasiliensis[J]. Journal of Tropical Biology, 2015, 6(2): 113-118. doi: 10.15886/j.cnki.rdswxb.2015.02.002
Citation: XIAO Hua, LIN Yunzheng, YAO Di, OUYANG Mo, HUANG Xi. Cloning and Characterization of a Zinc-finger Gene of Hevea brasiliensis[J]. Journal of Tropical Biology, 2015, 6(2): 113-118. doi: 10.15886/j.cnki.rdswxb.2015.02.002
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Cloning and Characterization of a Zinc-finger Gene of Hevea brasiliensis

doi: 10.15886/j.cnki.rdswxb.2015.02.002

  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agronomy, Hainan University, Haikou 570228, China

  • Received Date: 2015-03-15
  • Zinc-finger is a protein that binds DNA / RNA and regulates gene expression. In previous work,we identified a Zinc-finger protein（ termed as Hb Zinc-finger） that interacted with Hb JAZ1 in Y2 H against rubber latex c DNA yeast library. The full-length c DNA of Hb Zinc-finger gene is 2 262 bp in length and encodes 753 aa.C-terminal of Hb Zinc-finger contains a PB1 domain. A ZnF_ZZ domain exists between 336 aa and 381 aa. Subcellular fluorescent localization result showed that the Hb Zinc-finger was located in nucleus. Additionally,Hb Zinc-finger showed strong self transcriptional activation in yeast. Furthermore,the expression of Hb Zinc-finger was affected by continuously wounding in rubber latex,suggesting that Hb Zinc-finger is possibly regulated by the JA signaling pathway.
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Cloning and Characterization of a Zinc-finger Gene of Hevea brasiliensis

doi: 10.15886/j.cnki.rdswxb.2015.02.002
  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agronomy, Hainan University, Haikou 570228, China
  • Received Date: 2015-03-15

Abstract: Zinc-finger is a protein that binds DNA / RNA and regulates gene expression. In previous work,we identified a Zinc-finger protein（ termed as Hb Zinc-finger） that interacted with Hb JAZ1 in Y2 H against rubber latex c DNA yeast library. The full-length c DNA of Hb Zinc-finger gene is 2 262 bp in length and encodes 753 aa.C-terminal of Hb Zinc-finger contains a PB1 domain. A ZnF_ZZ domain exists between 336 aa and 381 aa. Subcellular fluorescent localization result showed that the Hb Zinc-finger was located in nucleus. Additionally,Hb Zinc-finger showed strong self transcriptional activation in yeast. Furthermore,the expression of Hb Zinc-finger was affected by continuously wounding in rubber latex,suggesting that Hb Zinc-finger is possibly regulated by the JA signaling pathway.

XIAO Hua, LIN Yunzheng, YAO Di, OUYANG Mo, HUANG Xi. Cloning and Characterization of a Zinc-finger Gene of Hevea brasiliensis[J]. Journal of Tropical Biology, 2015, 6(2): 113-118. doi: 10.15886/j.cnki.rdswxb.2015.02.002
Citation: XIAO Hua, LIN Yunzheng, YAO Di, OUYANG Mo, HUANG Xi. Cloning and Characterization of a Zinc-finger Gene of Hevea brasiliensis[J]. Journal of Tropical Biology, 2015, 6(2): 113-118. doi: 10.15886/j.cnki.rdswxb.2015.02.002
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