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Volume 5 Issue 1
Mar.  2014
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Article Navigation >  Journal of Tropical Biology  > 2014  >  5(1): 25-29

PAN Yanpeng, CAO Xianying, WU Zhaoying, CHANG Zhijie, WANG Yinyin, TIAN Bo, HAO Xinbao. The Effect of RNAi Targeting bcr/abl Fusion Gene on the Proliferation Activity of the Chronic Myelogenous Leukemia K562[J]. Journal of Tropical Biology, 2014, 5(1): 25-29. doi: 10.15886/j.cnki.rdswxb.2014.01.016
Citation: PAN Yanpeng, CAO Xianying, WU Zhaoying, CHANG Zhijie, WANG Yinyin, TIAN Bo, HAO Xinbao. The Effect of RNAi Targeting bcr/abl Fusion Gene on the Proliferation Activity of the Chronic Myelogenous Leukemia K562[J]. Journal of Tropical Biology, 2014, 5(1): 25-29. doi: 10.15886/j.cnki.rdswxb.2014.01.016
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The Effect of RNAi Targeting bcr/abl Fusion Gene on the Proliferation Activity of the Chronic Myelogenous Leukemia K562

doi: 10.15886/j.cnki.rdswxb.2014.01.016

  • 1. College of Materials Sciences and Chemical Engineering, Hainan University, Haikou 570228, China;

  • 2. School of Medicine/State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China;

  • 3. Department of Surgical Oncology, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712083, China;

  • 4. Affiliated Hospital of Hainan Medical College, Haikou 570228, China

  • Received Date: 2014-01-17
  • bcr /abl RNAi lentivirus vector was constructed and used to transfect K562 cells（ the first human immortalised myelogenous leukemia line to be established）,and the stable transfectants were selected. RNAi efficiency was assessed by Real-time PCR and Western blotting. The viability of the K562 cells was detected by CCK-8 assay and clonogenic assay. The results showed that bcr / abl RNAi significantly down regulates the amount of bcr / abl mRNA and P210bcr / abl fusion protein,and inhibits the proliferation of the K562 cells. The method of RNAi targeting bcr / abl fusion gene highlights a new way of chronic myelogenous leukemia treatment.
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The Effect of RNAi Targeting bcr/abl Fusion Gene on the Proliferation Activity of the Chronic Myelogenous Leukemia K562

doi: 10.15886/j.cnki.rdswxb.2014.01.016
  • 1. College of Materials Sciences and Chemical Engineering, Hainan University, Haikou 570228, China;
  • 2. School of Medicine/State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China;
  • 3. Department of Surgical Oncology, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712083, China;
  • 4. Affiliated Hospital of Hainan Medical College, Haikou 570228, China
  • Received Date: 2014-01-17

Abstract: bcr /abl RNAi lentivirus vector was constructed and used to transfect K562 cells（ the first human immortalised myelogenous leukemia line to be established）,and the stable transfectants were selected. RNAi efficiency was assessed by Real-time PCR and Western blotting. The viability of the K562 cells was detected by CCK-8 assay and clonogenic assay. The results showed that bcr / abl RNAi significantly down regulates the amount of bcr / abl mRNA and P210bcr / abl fusion protein,and inhibits the proliferation of the K562 cells. The method of RNAi targeting bcr / abl fusion gene highlights a new way of chronic myelogenous leukemia treatment.

PAN Yanpeng, CAO Xianying, WU Zhaoying, CHANG Zhijie, WANG Yinyin, TIAN Bo, HAO Xinbao. The Effect of RNAi Targeting bcr/abl Fusion Gene on the Proliferation Activity of the Chronic Myelogenous Leukemia K562[J]. Journal of Tropical Biology, 2014, 5(1): 25-29. doi: 10.15886/j.cnki.rdswxb.2014.01.016
Citation: PAN Yanpeng, CAO Xianying, WU Zhaoying, CHANG Zhijie, WANG Yinyin, TIAN Bo, HAO Xinbao. The Effect of RNAi Targeting bcr/abl Fusion Gene on the Proliferation Activity of the Chronic Myelogenous Leukemia K562[J]. Journal of Tropical Biology, 2014, 5(1): 25-29. doi: 10.15886/j.cnki.rdswxb.2014.01.016
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