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Volume 5 Issue 1
Mar.  2014
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Article Navigation >  Journal of Tropical Biology  > 2014  >  5(1): 67-72

LIU Xuehui, ZHANG Shixin, YANG Peng, TIAN Weimin. A Cryo-sectioning Method Used for Micro-structural Observation of Manihot esculenta Crantz[J]. Journal of Tropical Biology, 2014, 5(1): 67-72. doi: 10.15886/j.cnki.rdswxb.2014.01.010
Citation: LIU Xuehui, ZHANG Shixin, YANG Peng, TIAN Weimin. A Cryo-sectioning Method Used for Micro-structural Observation of Manihot esculenta Crantz[J]. Journal of Tropical Biology, 2014, 5(1): 67-72. doi: 10.15886/j.cnki.rdswxb.2014.01.010
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A Cryo-sectioning Method Used for Micro-structural Observation of Manihot esculenta Crantz

doi: 10.15886/j.cnki.rdswxb.2014.01.010

  • 1. Hainan University, Haikou 570228, China;

  • 2. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture/State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou 571737, China

  • Received Date: 2014-03-14
  • Cryo-sectioning,a fast and easy technique,is used to section delicate tissue and fleshy tissue and preserve cells within water-soluble and lipid-soluble inclusions and other features without dehydration and transparency,and has been widely used in animal and human studies,but seldom applied in plants. The roots,stems and leaves of cassava（ Manihot esculenta Crantz.） were sectioned by using direct embedding cryosectioning,sucrose cryoprotectant cryosectioning and paraffin sectioning and observed and photographed under microscope. The results showed that the microstructures of the roots,stems and leaves of cassava sectioned by using the sucrose cryoprotectant cryosectioning were observed vivid to the true with better structural integrity and images of the sectioned tissues than those from the other two sectioning methods.
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A Cryo-sectioning Method Used for Micro-structural Observation of Manihot esculenta Crantz

doi: 10.15886/j.cnki.rdswxb.2014.01.010
  • 1. Hainan University, Haikou 570228, China;
  • 2. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture/State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou 571737, China
  • Received Date: 2014-03-14

Abstract: Cryo-sectioning,a fast and easy technique,is used to section delicate tissue and fleshy tissue and preserve cells within water-soluble and lipid-soluble inclusions and other features without dehydration and transparency,and has been widely used in animal and human studies,but seldom applied in plants. The roots,stems and leaves of cassava（ Manihot esculenta Crantz.） were sectioned by using direct embedding cryosectioning,sucrose cryoprotectant cryosectioning and paraffin sectioning and observed and photographed under microscope. The results showed that the microstructures of the roots,stems and leaves of cassava sectioned by using the sucrose cryoprotectant cryosectioning were observed vivid to the true with better structural integrity and images of the sectioned tissues than those from the other two sectioning methods.

LIU Xuehui, ZHANG Shixin, YANG Peng, TIAN Weimin. A Cryo-sectioning Method Used for Micro-structural Observation of Manihot esculenta Crantz[J]. Journal of Tropical Biology, 2014, 5(1): 67-72. doi: 10.15886/j.cnki.rdswxb.2014.01.010
Citation: LIU Xuehui, ZHANG Shixin, YANG Peng, TIAN Weimin. A Cryo-sectioning Method Used for Micro-structural Observation of Manihot esculenta Crantz[J]. Journal of Tropical Biology, 2014, 5(1): 67-72. doi: 10.15886/j.cnki.rdswxb.2014.01.010
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