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Volume 5 Issue 1
Mar.  2014
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Article Navigation >  Journal of Tropical Biology  > 2014  >  5(1): 30-35

GAO Zhiliang, XIAO Xiaorong, CHEN Yan, LI Xiuqiong, FU Xiumei, LIN Daozhe, CHEN Yinhua. Isolation and Expression Profiling of Rice OsNHO1 Full-length cDNA[J]. Journal of Tropical Biology, 2014, 5(1): 30-35. doi: 10.15886/j.cnki.rdswxb.2014.01.001
Citation: GAO Zhiliang, XIAO Xiaorong, CHEN Yan, LI Xiuqiong, FU Xiumei, LIN Daozhe, CHEN Yinhua. Isolation and Expression Profiling of Rice OsNHO1 Full-length cDNA[J]. Journal of Tropical Biology, 2014, 5(1): 30-35. doi: 10.15886/j.cnki.rdswxb.2014.01.001
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Isolation and Expression Profiling of Rice OsNHO1 Full-length cDNA

doi: 10.15886/j.cnki.rdswxb.2014.01.001

  • 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou 570228, China;

  • 2. College of Agronomy, Hainan University, Haikou 570228, China;

  • 3. Beijing University of Agriculture, Beijing 102206, China

  • Received Date: 2014-02-26
  • Arabidopsis non-host resistance gene（ AtNHO1） encodes a putative glycerol kinase and plays an important role in plant PAMPs triggered immunity（ PTI）. The full length cDNA of rice（ Oryza sativa） gene OsNHO1 was isolated by reverse transcription PCR,and the sequencing result showed that the open reading frame of OsNHO1 is 1 590 bp,encoding a 529 amino acids with a conserved NBD-sugar-kinase domain similar with NHO1 from Arabidopsis. Bioinformatics analysis showed that the amino acid sequence of the OsNHO1 is 99% similar with that of the NHO1 from Arabidopsis thaliana. Semi-quantitative RT-PCR analysis of the expression of OsNHO1 induced by SA and PXO999 showed that the OsNHO1 gene expression was induced by SA,PXO99,indicating that OsNHO1 is related to SA-mediated signaling transduction pathway and plays a key role in rice PAMPs triggered immunity（ PTI）.
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Isolation and Expression Profiling of Rice OsNHO1 Full-length cDNA

doi: 10.15886/j.cnki.rdswxb.2014.01.001
  • 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou 570228, China;
  • 2. College of Agronomy, Hainan University, Haikou 570228, China;
  • 3. Beijing University of Agriculture, Beijing 102206, China
  • Received Date: 2014-02-26

Abstract: Arabidopsis non-host resistance gene（ AtNHO1） encodes a putative glycerol kinase and plays an important role in plant PAMPs triggered immunity（ PTI）. The full length cDNA of rice（ Oryza sativa） gene OsNHO1 was isolated by reverse transcription PCR,and the sequencing result showed that the open reading frame of OsNHO1 is 1 590 bp,encoding a 529 amino acids with a conserved NBD-sugar-kinase domain similar with NHO1 from Arabidopsis. Bioinformatics analysis showed that the amino acid sequence of the OsNHO1 is 99% similar with that of the NHO1 from Arabidopsis thaliana. Semi-quantitative RT-PCR analysis of the expression of OsNHO1 induced by SA and PXO999 showed that the OsNHO1 gene expression was induced by SA,PXO99,indicating that OsNHO1 is related to SA-mediated signaling transduction pathway and plays a key role in rice PAMPs triggered immunity（ PTI）.

GAO Zhiliang, XIAO Xiaorong, CHEN Yan, LI Xiuqiong, FU Xiumei, LIN Daozhe, CHEN Yinhua. Isolation and Expression Profiling of Rice OsNHO1 Full-length cDNA[J]. Journal of Tropical Biology, 2014, 5(1): 30-35. doi: 10.15886/j.cnki.rdswxb.2014.01.001
Citation: GAO Zhiliang, XIAO Xiaorong, CHEN Yan, LI Xiuqiong, FU Xiumei, LIN Daozhe, CHEN Yinhua. Isolation and Expression Profiling of Rice OsNHO1 Full-length cDNA[J]. Journal of Tropical Biology, 2014, 5(1): 30-35. doi: 10.15886/j.cnki.rdswxb.2014.01.001
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