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Volume 4 Issue 4
Dec.  2013
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(4): 335-341

HOU Jie, LIU Xuebing, CAO Feng, PAN Yanpeng, LÜ Yang, WU Ziyang, ZHENG Yusheng, CAO Xianying. Inhibition and Inducing of k562 Cells by Methallyl Isothiocyanate Extracted from Mustard Seeds[J]. Journal of Tropical Biology, 2013, 4(4): 335-341. doi: 10.15886/j.cnki.rdswxb.2013.04.018
Citation: HOU Jie, LIU Xuebing, CAO Feng, PAN Yanpeng, LÜ Yang, WU Ziyang, ZHENG Yusheng, CAO Xianying. Inhibition and Inducing of k562 Cells by Methallyl Isothiocyanate Extracted from Mustard Seeds[J]. Journal of Tropical Biology, 2013, 4(4): 335-341. doi: 10.15886/j.cnki.rdswxb.2013.04.018
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Inhibition and Inducing of k562 Cells by Methallyl Isothiocyanate Extracted from Mustard Seeds

doi: 10.15886/j.cnki.rdswxb.2013.04.018

  • 1. Ministry of Education Key Laboratory for Advanced Materials in Tropical Island Resources, Hainan University, Haikou 570228, China;

  • 2. College of Material and Chemical Engineering, Hainan University, Haikou 570228, China

  • Received Date: 2013-11-10
  • Methallyl isothiocyanate（ MITC） were extracted from the seeds of mustard（ Brassica juncea）,a cruciferous plant,which grows in Hainan Province,China. The inhibition effect of MITC on proliferation of k562 cells was determined by a MTT assay,and the cell morphology was observed by using Wright-giemsa,DAPI and Rodanmin 123 staining. MITC showed a concentration-response relationship with k562 cells in cell proliferation in vitro. The k562 cells were inhibited at the highest rate（ 87. 5 ± 1. 4） % when treated with the MITC at 120 μmol·L-1 for 96 h. The k562 cells treated with MITC for 24,48,72,96,120 h gave respective IC50 of 250. 007,100. 584,39. 030,54. 701,88. 719 μmol L-1. And the cells shrank with nuclear chromatin condensation,nuclear inward depression and low mitochondrial potential. These morphological changes suggest that k562 cell apoptosis were induced by MITC.
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Inhibition and Inducing of k562 Cells by Methallyl Isothiocyanate Extracted from Mustard Seeds

doi: 10.15886/j.cnki.rdswxb.2013.04.018
  • 1. Ministry of Education Key Laboratory for Advanced Materials in Tropical Island Resources, Hainan University, Haikou 570228, China;
  • 2. College of Material and Chemical Engineering, Hainan University, Haikou 570228, China
  • Received Date: 2013-11-10

Abstract: Methallyl isothiocyanate（ MITC） were extracted from the seeds of mustard（ Brassica juncea）,a cruciferous plant,which grows in Hainan Province,China. The inhibition effect of MITC on proliferation of k562 cells was determined by a MTT assay,and the cell morphology was observed by using Wright-giemsa,DAPI and Rodanmin 123 staining. MITC showed a concentration-response relationship with k562 cells in cell proliferation in vitro. The k562 cells were inhibited at the highest rate（ 87. 5 ± 1. 4） % when treated with the MITC at 120 μmol·L-1 for 96 h. The k562 cells treated with MITC for 24,48,72,96,120 h gave respective IC50 of 250. 007,100. 584,39. 030,54. 701,88. 719 μmol L-1. And the cells shrank with nuclear chromatin condensation,nuclear inward depression and low mitochondrial potential. These morphological changes suggest that k562 cell apoptosis were induced by MITC.

HOU Jie, LIU Xuebing, CAO Feng, PAN Yanpeng, LÜ Yang, WU Ziyang, ZHENG Yusheng, CAO Xianying. Inhibition and Inducing of k562 Cells by Methallyl Isothiocyanate Extracted from Mustard Seeds[J]. Journal of Tropical Biology, 2013, 4(4): 335-341. doi: 10.15886/j.cnki.rdswxb.2013.04.018
Citation: HOU Jie, LIU Xuebing, CAO Feng, PAN Yanpeng, LÜ Yang, WU Ziyang, ZHENG Yusheng, CAO Xianying. Inhibition and Inducing of k562 Cells by Methallyl Isothiocyanate Extracted from Mustard Seeds[J]. Journal of Tropical Biology, 2013, 4(4): 335-341. doi: 10.15886/j.cnki.rdswxb.2013.04.018
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