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Volume 4 Issue 4
Dec.  2013
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(4): 332-334,346

GUO Guiying, LI Xiang, LI Dan, LIAO Chenghong, YANG Nuo, ZENG Jifeng, ZHENG Jiping, ZHANG Liling, YU Jinan. Isolation and Identification of Klebsiella pneumonia from the blood of a farmed Python molurus bivittatus infected with pneumonia[J]. Journal of Tropical Biology, 2013, 4(4): 332-334,346. doi: 10.15886/j.cnki.rdswxb.2013.04.017
Citation: GUO Guiying, LI Xiang, LI Dan, LIAO Chenghong, YANG Nuo, ZENG Jifeng, ZHENG Jiping, ZHANG Liling, YU Jinan. Isolation and Identification of Klebsiella pneumonia from the blood of a farmed Python molurus bivittatus infected with pneumonia[J]. Journal of Tropical Biology, 2013, 4(4): 332-334,346. doi: 10.15886/j.cnki.rdswxb.2013.04.017
shu

Isolation and Identification of Klebsiella pneumonia from the blood of a farmed Python molurus bivittatus infected with pneumonia

doi: 10.15886/j.cnki.rdswxb.2013.04.017

  • 1. Academic Affairs Office, Hainan University, Haikou 570228, China;

  • 2. College of Agronomy, Hainan University, Haikou 570228, China;

  • 3. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources:State Key Laboratory Breeding Base, Haikou 570228, China;

  • 4. Ministry of Education Key Laboratory of Tropical Biological Resources, Hainan University, Haikou 570228, China;

  • 5. Ministry of Education Key Laboratory for Conservation, Development and Utilization of Tropical Crop Germplasm Resources, Hainan University, Haikou 570228, China;

  • 6. College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China

  • Received Date: 2013-11-28
  • Python molurus,as a large nonvenomous python species found in many tropical and subtropical forests,has been farmed for its high value in fine skin,nutrition and medicine in recent years. But pneumonia,the most serious and the most common disease of python,has caused death of python at a high rate. Blood sample was collected from a farmed Burmese python（ Python molurus bivittatus） with pneumonia to determine the pathogens of pneumonia. The specimens were incubated at 33 ℃ for 48 h to produce colony growth under the aerobic condition or with 5% CO2 in bacterial,actinomycetal and fungal mediums,separately. The isolated colonies were then characterized by gram staining and resorted to 16S rRNA gene sequencing and GenBank BLAST. The result showed that the isolates from the python was identified to be Klebsiella pneumonia.
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Isolation and Identification of Klebsiella pneumonia from the blood of a farmed Python molurus bivittatus infected with pneumonia

doi: 10.15886/j.cnki.rdswxb.2013.04.017
  • 1. Academic Affairs Office, Hainan University, Haikou 570228, China;
  • 2. College of Agronomy, Hainan University, Haikou 570228, China;
  • 3. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources:State Key Laboratory Breeding Base, Haikou 570228, China;
  • 4. Ministry of Education Key Laboratory of Tropical Biological Resources, Hainan University, Haikou 570228, China;
  • 5. Ministry of Education Key Laboratory for Conservation, Development and Utilization of Tropical Crop Germplasm Resources, Hainan University, Haikou 570228, China;
  • 6. College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China
  • Received Date: 2013-11-28

Abstract: Python molurus,as a large nonvenomous python species found in many tropical and subtropical forests,has been farmed for its high value in fine skin,nutrition and medicine in recent years. But pneumonia,the most serious and the most common disease of python,has caused death of python at a high rate. Blood sample was collected from a farmed Burmese python（ Python molurus bivittatus） with pneumonia to determine the pathogens of pneumonia. The specimens were incubated at 33 ℃ for 48 h to produce colony growth under the aerobic condition or with 5% CO2 in bacterial,actinomycetal and fungal mediums,separately. The isolated colonies were then characterized by gram staining and resorted to 16S rRNA gene sequencing and GenBank BLAST. The result showed that the isolates from the python was identified to be Klebsiella pneumonia.

GUO Guiying, LI Xiang, LI Dan, LIAO Chenghong, YANG Nuo, ZENG Jifeng, ZHENG Jiping, ZHANG Liling, YU Jinan. Isolation and Identification of Klebsiella pneumonia from the blood of a farmed Python molurus bivittatus infected with pneumonia[J]. Journal of Tropical Biology, 2013, 4(4): 332-334,346. doi: 10.15886/j.cnki.rdswxb.2013.04.017
Citation: GUO Guiying, LI Xiang, LI Dan, LIAO Chenghong, YANG Nuo, ZENG Jifeng, ZHENG Jiping, ZHANG Liling, YU Jinan. Isolation and Identification of Klebsiella pneumonia from the blood of a farmed Python molurus bivittatus infected with pneumonia[J]. Journal of Tropical Biology, 2013, 4(4): 332-334,346. doi: 10.15886/j.cnki.rdswxb.2013.04.017
shu

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