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Volume 4 Issue 4
Dec.  2013
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(4): 369-373

ZHANG Han, ZHOU Yongcan, ZHAO Guangjun, CAI Yan, XIE Zhenyu, GUO Weiliang, WANG Shifeng. Establishment of Spectrophotometric Method for Concentration Determining of the Inactivated Vaccine Against Streptococcus agalactiae[J]. Journal of Tropical Biology, 2013, 4(4): 369-373. doi: 10.15886/j.cnki.rdswxb.2013.04.016
Citation: ZHANG Han, ZHOU Yongcan, ZHAO Guangjun, CAI Yan, XIE Zhenyu, GUO Weiliang, WANG Shifeng. Establishment of Spectrophotometric Method for Concentration Determining of the Inactivated Vaccine Against Streptococcus agalactiae[J]. Journal of Tropical Biology, 2013, 4(4): 369-373. doi: 10.15886/j.cnki.rdswxb.2013.04.016
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Establishment of Spectrophotometric Method for Concentration Determining of the Inactivated Vaccine Against Streptococcus agalactiae

doi: 10.15886/j.cnki.rdswxb.2013.04.016

  • College of Marine Sciences, Hainan University/State Key Laboratory Breeding Base for Sustainable Utilization of Tropical Bioresource, Haikou 570228, China

  • Received Date: 2013-08-13
  • A method for rapid determination of the concentration of inactivated vaccine against the bacteria, Streptococcus agalactiae was established by spectrophotometry. At the wavelength of 600 nm both physiological saline and inactivated vaccine showed smooth absorbance change and no characteristic absorption peak wavelength. The A600 value（ X） was positively correlated with the concentration of the vaccine（ Y）（ r = 0. 998 3, df = 6,P ＜ 0. 01）,as revealed by the equation y = 11. 277x- 2. 022 9; the A600 value of vaccine remained stable when it was inactivated by 0. 4% formaldehyde for 3 h. The determination was repeated by 4 laboratory technicians,and the results showed the A600 value was in accordance with the above correlation equation and its RSD ranged between 0. 5% —1. 2%. This indicates that this method for determination of the concentration of inactivated vaccine against the bacteria was very stable,repeatable,easy to handle and rapid.
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Establishment of Spectrophotometric Method for Concentration Determining of the Inactivated Vaccine Against Streptococcus agalactiae

doi: 10.15886/j.cnki.rdswxb.2013.04.016
  • College of Marine Sciences, Hainan University/State Key Laboratory Breeding Base for Sustainable Utilization of Tropical Bioresource, Haikou 570228, China
  • Received Date: 2013-08-13

Abstract: A method for rapid determination of the concentration of inactivated vaccine against the bacteria, Streptococcus agalactiae was established by spectrophotometry. At the wavelength of 600 nm both physiological saline and inactivated vaccine showed smooth absorbance change and no characteristic absorption peak wavelength. The A600 value（ X） was positively correlated with the concentration of the vaccine（ Y）（ r = 0. 998 3, df = 6,P ＜ 0. 01）,as revealed by the equation y = 11. 277x- 2. 022 9; the A600 value of vaccine remained stable when it was inactivated by 0. 4% formaldehyde for 3 h. The determination was repeated by 4 laboratory technicians,and the results showed the A600 value was in accordance with the above correlation equation and its RSD ranged between 0. 5% —1. 2%. This indicates that this method for determination of the concentration of inactivated vaccine against the bacteria was very stable,repeatable,easy to handle and rapid.

ZHANG Han, ZHOU Yongcan, ZHAO Guangjun, CAI Yan, XIE Zhenyu, GUO Weiliang, WANG Shifeng. Establishment of Spectrophotometric Method for Concentration Determining of the Inactivated Vaccine Against Streptococcus agalactiae[J]. Journal of Tropical Biology, 2013, 4(4): 369-373. doi: 10.15886/j.cnki.rdswxb.2013.04.016
Citation: ZHANG Han, ZHOU Yongcan, ZHAO Guangjun, CAI Yan, XIE Zhenyu, GUO Weiliang, WANG Shifeng. Establishment of Spectrophotometric Method for Concentration Determining of the Inactivated Vaccine Against Streptococcus agalactiae[J]. Journal of Tropical Biology, 2013, 4(4): 369-373. doi: 10.15886/j.cnki.rdswxb.2013.04.016
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