In-vitro rapid propagation of Dendrobium officinale Kimura et Migo

Key words: 
• Dendrobium officinale Kimura et Migo /
• protocom-like body /
• in-vitro rapid propagation

Abstract: The immature seeds of Dendrobium officinale Kimura et Migo were used as explants. The explants were cultured on media supplemented with such factors as coconut water（ CW）,potato puree and NAA（ all with different concentrations） to investigate the effect of these factors on proliferation culture of D. officinale Kimura et Migo. The explants cultured on 1 /2 MS + φ（ CW） = 10% + NAA 0. 1 mg L^-1 produced most protocom-like bodies with a multiplication coefficient being as high as 145. 28. The plantlets were not sensitive to the changes of the concentrations of exogenous auxins,such as IBA,NAA and IAA at the stage of invigorating culture,and no obvious difference was observed in plant growth vigor in the treatments at the concentrations ranging from 0 to 2. 50 mg L^-1 . However,the plantlets treated with IBA at 0. 75 mg·L^-1or NAA at 0. 5 mg·L^-1 grew better in vigor and uniform among individuals. The plantlets cultured on the rooting medium 1 /2 MS supplemented with w = 10% banana puree and NAA 1. 0mg L^-1 produced better roots and grew stronger.