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Volume 4 Issue 4
Dec.  2013
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(4): 313-316

WU Yuanli, TAN Xianjiao, ZHANG Shuzhen, YANG Benpeng. Control of Browning in Tissue Culture of Sugarcane（ Saccharum officinarum L. ）[J]. Journal of Tropical Biology, 2013, 4(4): 313-316. doi: 10.15886/j.cnki.rdswxb.2013.04.011
Citation: WU Yuanli, TAN Xianjiao, ZHANG Shuzhen, YANG Benpeng. Control of Browning in Tissue Culture of Sugarcane（ Saccharum officinarum L. ）[J]. Journal of Tropical Biology, 2013, 4(4): 313-316. doi: 10.15886/j.cnki.rdswxb.2013.04.011
shu

Control of Browning in Tissue Culture of Sugarcane（ Saccharum officinarum L. ）

doi: 10.15886/j.cnki.rdswxb.2013.04.011

  • Sugarcane Research Center of Chinese Academy of Tropical Agricultural Sciences/Institute of Tropical Bioscience and Biotechnology of Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, China

  • Received Date: 2013-09-26
  • Browning is a restraint for mass propatation of sugarcane by tissue culture. For successful tissue culture of sugarcane it is very important to inhibit browning of sugarcane under tissue culture and make sure the tips of sugarcane stem grow and differentiate well. In this experiment axillary buds at different positions of stem sugarcane were used as explants and pretreated with different antioxidants,ie.,ascorbic acid（ VitC）,PVP, Na2S2O3 ·5H2O. The results showed that the axillary buds germinated from the young canes grew well and can be a good choice for explants,and that the pretreatment of the explants with VitC（ 0. 75 g·L-1） controlled browning of sugarcane efficiently and did not give impacts on the growth of the explants.
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Control of Browning in Tissue Culture of Sugarcane（ Saccharum officinarum L. ）

doi: 10.15886/j.cnki.rdswxb.2013.04.011
  • Sugarcane Research Center of Chinese Academy of Tropical Agricultural Sciences/Institute of Tropical Bioscience and Biotechnology of Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, China
  • Received Date: 2013-09-26

Abstract: Browning is a restraint for mass propatation of sugarcane by tissue culture. For successful tissue culture of sugarcane it is very important to inhibit browning of sugarcane under tissue culture and make sure the tips of sugarcane stem grow and differentiate well. In this experiment axillary buds at different positions of stem sugarcane were used as explants and pretreated with different antioxidants,ie.,ascorbic acid（ VitC）,PVP, Na2S2O3 ·5H2O. The results showed that the axillary buds germinated from the young canes grew well and can be a good choice for explants,and that the pretreatment of the explants with VitC（ 0. 75 g·L-1） controlled browning of sugarcane efficiently and did not give impacts on the growth of the explants.

WU Yuanli, TAN Xianjiao, ZHANG Shuzhen, YANG Benpeng. Control of Browning in Tissue Culture of Sugarcane（ Saccharum officinarum L. ）[J]. Journal of Tropical Biology, 2013, 4(4): 313-316. doi: 10.15886/j.cnki.rdswxb.2013.04.011
Citation: WU Yuanli, TAN Xianjiao, ZHANG Shuzhen, YANG Benpeng. Control of Browning in Tissue Culture of Sugarcane（ Saccharum officinarum L. ）[J]. Journal of Tropical Biology, 2013, 4(4): 313-316. doi: 10.15886/j.cnki.rdswxb.2013.04.011
shu

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