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Volume 4 Issue 2
Apr.  2025
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(2): 198-202

ZHAO Yiluan, HUANG Qin, JIA Xian, WANG Ying, WU Kunxin, CHEN Xiongting. Preparation of Microscopic Slides of Oncidium for Chromosome Observation[J]. Journal of Tropical Biology, 2013, 4(2): 198-202. doi: 10.15886/j.cnki.rdswxb.2013.02.016
Citation: ZHAO Yiluan, HUANG Qin, JIA Xian, WANG Ying, WU Kunxin, CHEN Xiongting. Preparation of Microscopic Slides of Oncidium for Chromosome Observation[J]. Journal of Tropical Biology, 2013, 4(2): 198-202. doi: 10.15886/j.cnki.rdswxb.2013.02.016
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Preparation of Microscopic Slides of Oncidium for Chromosome Observation

doi: 10.15886/j.cnki.rdswxb.2013.02.016

  • 1. College of Agronomy, Hainan University, Haikou 570228, China;

  • 2. Ministry of Agriculture Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agri- cultural Sciences, Haikou 571101, China

  • Received Date: 2013-03-06
  • The root tips of Oncidium flexuosum Lodd were used to study the effects of sampling time,pretreatment chemical solution,fixed solutions,hydrolyzing time and staining on the chromosome observation slides. The results showed that the optimal temperature for tissue culture of the Oncidium was at 25 ℃ and that the optimal sampling time of root tip was about 10: 00 a. m in May to June. The samples were pretreated with a solution of 0. 1% colchicine and 0. 002 mol·L-1 8-hydroxyquinoline at room temperature for 4 hours,and fixed with the Carnoy’s fixative for 24 hours,and then hydrolyzed with 1 mol·L-1 HCl for 8 min at 60 ℃ and stained with modified Carbol fuchsin solution. The slides thus prepared for chromosome observation were proved to be optimum.
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Preparation of Microscopic Slides of Oncidium for Chromosome Observation

doi: 10.15886/j.cnki.rdswxb.2013.02.016
  • 1. College of Agronomy, Hainan University, Haikou 570228, China;
  • 2. Ministry of Agriculture Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agri- cultural Sciences, Haikou 571101, China
  • Received Date: 2013-03-06

Abstract: The root tips of Oncidium flexuosum Lodd were used to study the effects of sampling time,pretreatment chemical solution,fixed solutions,hydrolyzing time and staining on the chromosome observation slides. The results showed that the optimal temperature for tissue culture of the Oncidium was at 25 ℃ and that the optimal sampling time of root tip was about 10: 00 a. m in May to June. The samples were pretreated with a solution of 0. 1% colchicine and 0. 002 mol·L-1 8-hydroxyquinoline at room temperature for 4 hours,and fixed with the Carnoy’s fixative for 24 hours,and then hydrolyzed with 1 mol·L-1 HCl for 8 min at 60 ℃ and stained with modified Carbol fuchsin solution. The slides thus prepared for chromosome observation were proved to be optimum.

ZHAO Yiluan, HUANG Qin, JIA Xian, WANG Ying, WU Kunxin, CHEN Xiongting. Preparation of Microscopic Slides of Oncidium for Chromosome Observation[J]. Journal of Tropical Biology, 2013, 4(2): 198-202. doi: 10.15886/j.cnki.rdswxb.2013.02.016
Citation: ZHAO Yiluan, HUANG Qin, JIA Xian, WANG Ying, WU Kunxin, CHEN Xiongting. Preparation of Microscopic Slides of Oncidium for Chromosome Observation[J]. Journal of Tropical Biology, 2013, 4(2): 198-202. doi: 10.15886/j.cnki.rdswxb.2013.02.016
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