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Volume 4 Issue 2
Apr.  2025
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(2): 146-152,159

WANG Defeng, CHEN Yan, XIAO Wenfang, FU Xiumei, XIAO Xiaorong, LI Xiuqiong, PANG Jinhuan, CHEN Yinhua. Obtaining and the Preliminary Analysis of Rice OsCERK2 Transgenic Plants[J]. Journal of Tropical Biology, 2013, 4(2): 146-152,159. doi: 10.15886/j.cnki.rdswxb.2013.02.009
Citation: WANG Defeng, CHEN Yan, XIAO Wenfang, FU Xiumei, XIAO Xiaorong, LI Xiuqiong, PANG Jinhuan, CHEN Yinhua. Obtaining and the Preliminary Analysis of Rice OsCERK2 Transgenic Plants[J]. Journal of Tropical Biology, 2013, 4(2): 146-152,159. doi: 10.15886/j.cnki.rdswxb.2013.02.009
shu

Obtaining and the Preliminary Analysis of Rice OsCERK2 Transgenic Plants

doi: 10.15886/j.cnki.rdswxb.2013.02.009

  • 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou 570228, China;

  • 2. Institute Of Microbiology Chinese Academy of Sciences, Beijing 10010, China;

  • 3. Agriculture College of Hainan University, Haikou 570228, China

  • Received Date: 2013-04-11
  • In this study,Oryza sativa ssp. japonica var. Nipponbare used as material,the Arabidopsis CERK1 was used to retrieve its homologous genes in the rice genome and a rice homologous gene CERK2,OsCERK2 was obtained,which cDNA of OsCERK2 was isolated by RT-PCR. Bioinformatics analysis showed that OsCERK2 was a plasma membrane protein including a signal peptide,its extracellular domain included an LsyM domain,its kinase domain included a protein tyrosine kinase domain. Overexpression vector driven by 35S promoter and RNA interference （RNAi） vector driven by maize ubiquitin promoter were constructed. The OsCERK2 gene was then transferred into rice by Agrobacterium-mediated transformation. T0 transgenic plants were obtained from each vector. Amplifying T0 transgenic plants by PCR and RT-PCR showed transgenic plants with efficient expression were gained. The results would lay a foundation on further studying functions of OsCERK2.
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Obtaining and the Preliminary Analysis of Rice OsCERK2 Transgenic Plants

doi: 10.15886/j.cnki.rdswxb.2013.02.009
  • 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou 570228, China;
  • 2. Institute Of Microbiology Chinese Academy of Sciences, Beijing 10010, China;
  • 3. Agriculture College of Hainan University, Haikou 570228, China
  • Received Date: 2013-04-11

Abstract: In this study,Oryza sativa ssp. japonica var. Nipponbare used as material,the Arabidopsis CERK1 was used to retrieve its homologous genes in the rice genome and a rice homologous gene CERK2,OsCERK2 was obtained,which cDNA of OsCERK2 was isolated by RT-PCR. Bioinformatics analysis showed that OsCERK2 was a plasma membrane protein including a signal peptide,its extracellular domain included an LsyM domain,its kinase domain included a protein tyrosine kinase domain. Overexpression vector driven by 35S promoter and RNA interference （RNAi） vector driven by maize ubiquitin promoter were constructed. The OsCERK2 gene was then transferred into rice by Agrobacterium-mediated transformation. T0 transgenic plants were obtained from each vector. Amplifying T0 transgenic plants by PCR and RT-PCR showed transgenic plants with efficient expression were gained. The results would lay a foundation on further studying functions of OsCERK2.

WANG Defeng, CHEN Yan, XIAO Wenfang, FU Xiumei, XIAO Xiaorong, LI Xiuqiong, PANG Jinhuan, CHEN Yinhua. Obtaining and the Preliminary Analysis of Rice OsCERK2 Transgenic Plants[J]. Journal of Tropical Biology, 2013, 4(2): 146-152,159. doi: 10.15886/j.cnki.rdswxb.2013.02.009
Citation: WANG Defeng, CHEN Yan, XIAO Wenfang, FU Xiumei, XIAO Xiaorong, LI Xiuqiong, PANG Jinhuan, CHEN Yinhua. Obtaining and the Preliminary Analysis of Rice OsCERK2 Transgenic Plants[J]. Journal of Tropical Biology, 2013, 4(2): 146-152,159. doi: 10.15886/j.cnki.rdswxb.2013.02.009
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