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Volume 4 Issue 2
Apr.  2025
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(2): 133-137,145

CAO Yuxin, HUA Long, ZHAO Tianlong, LI Mingrong, XIA Zhihui. Construction of Vector Expressing Xa21 during Whole Growth Stage[J]. Journal of Tropical Biology, 2013, 4(2): 133-137,145. doi: 10.15886/j.cnki.rdswxb.2013.02.001
Citation: CAO Yuxin, HUA Long, ZHAO Tianlong, LI Mingrong, XIA Zhihui. Construction of Vector Expressing Xa21 during Whole Growth Stage[J]. Journal of Tropical Biology, 2013, 4(2): 133-137,145. doi: 10.15886/j.cnki.rdswxb.2013.02.001
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Construction of Vector Expressing Xa21 during Whole Growth Stage

doi: 10.15886/j.cnki.rdswxb.2013.02.001

  • College of Agriculture, Hainan University, Haikou 570228, China

  • Received Date: 2013-04-25
  • In this study,the results of realtime PCR of rice sucrose synthase gene 1（RSuS1） showed that RSuS1 was highly expressed in sheath and leaf during whole growth stage. The promoter of RSuS1 was amplified from genomic DNA template of an indica rice variety"Minghui 63",then the DNA fragment of Xa21 including open reading frame and terminator was amplified from vector pDBXa21 carrying completely genomic Xa21 fragment. In order to breed transgenic rice with resistance to bacterial leaf blight during whole growth stage and without risk of comestible safety,we constructed an expression vector that Xa21 was driven by RSP.
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Construction of Vector Expressing Xa21 during Whole Growth Stage

doi: 10.15886/j.cnki.rdswxb.2013.02.001
  • College of Agriculture, Hainan University, Haikou 570228, China
  • Received Date: 2013-04-25

Abstract: In this study,the results of realtime PCR of rice sucrose synthase gene 1（RSuS1） showed that RSuS1 was highly expressed in sheath and leaf during whole growth stage. The promoter of RSuS1 was amplified from genomic DNA template of an indica rice variety"Minghui 63",then the DNA fragment of Xa21 including open reading frame and terminator was amplified from vector pDBXa21 carrying completely genomic Xa21 fragment. In order to breed transgenic rice with resistance to bacterial leaf blight during whole growth stage and without risk of comestible safety,we constructed an expression vector that Xa21 was driven by RSP.

CAO Yuxin, HUA Long, ZHAO Tianlong, LI Mingrong, XIA Zhihui. Construction of Vector Expressing Xa21 during Whole Growth Stage[J]. Journal of Tropical Biology, 2013, 4(2): 133-137,145. doi: 10.15886/j.cnki.rdswxb.2013.02.001
Citation: CAO Yuxin, HUA Long, ZHAO Tianlong, LI Mingrong, XIA Zhihui. Construction of Vector Expressing Xa21 during Whole Growth Stage[J]. Journal of Tropical Biology, 2013, 4(2): 133-137,145. doi: 10.15886/j.cnki.rdswxb.2013.02.001
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