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Volume 4 Issue 1
Jan.  2013
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(1): 36-44,55

FAN Lanyan, HE Chunping, LIANG Yanqiong, ZHENG Xiaolan, WU Weihuai, LI Rui, ZHENG Fucong. Characteristics of the Antimicrobial Active Component of Bacillus subtilis Strain Czk1[J]. Journal of Tropical Biology, 2013, 4(1): 36-44,55. doi: 10.15886/j.cnki.rdswxb.2013.01.007
Citation: FAN Lanyan, HE Chunping, LIANG Yanqiong, ZHENG Xiaolan, WU Weihuai, LI Rui, ZHENG Fucong. Characteristics of the Antimicrobial Active Component of Bacillus subtilis Strain Czk1[J]. Journal of Tropical Biology, 2013, 4(1): 36-44,55. doi: 10.15886/j.cnki.rdswxb.2013.01.007
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Characteristics of the Antimicrobial Active Component of Bacillus subtilis Strain Czk1

doi: 10.15886/j.cnki.rdswxb.2013.01.007

  • 1. College of Environment and Plant Protection, Hainan University, Danzhou 571737, China;

  • 2. Environment and Plant Protection Institute, CATAS/Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Danzhou 571737, China;

  • 3. Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture, Danzhou 571737, China;

  • 4. Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Danzhou 571737, China

  • Received Date: 2012-12-22
  • Cup-plate methods and plate growth rate method were used to determine antimicrobial substances of strain Czk1 of Bacillus substilis in the fermentation broth. The results indicated that the active component of this strain were extracellular metabolites and existed in the supernatant. Physicochemical analysis showed this antimicrobial component was not tolerant to high temperature and high and low acidity,and not sensitive to ultraviolet radiation,protease K,5 common metal ions and organic solvent. This component could be extracted by acid precipitation and methanol extraction. Single factor experiment showed this strain had the highest pathogen antagonistic activity when shake cultured at 28 ℃ and 180 r·min-1 for 3d on YPD medium inoculated at the rate of 7% and the initial pH7.
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Characteristics of the Antimicrobial Active Component of Bacillus subtilis Strain Czk1

doi: 10.15886/j.cnki.rdswxb.2013.01.007
  • 1. College of Environment and Plant Protection, Hainan University, Danzhou 571737, China;
  • 2. Environment and Plant Protection Institute, CATAS/Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Danzhou 571737, China;
  • 3. Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture, Danzhou 571737, China;
  • 4. Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Danzhou 571737, China
  • Received Date: 2012-12-22

Abstract: Cup-plate methods and plate growth rate method were used to determine antimicrobial substances of strain Czk1 of Bacillus substilis in the fermentation broth. The results indicated that the active component of this strain were extracellular metabolites and existed in the supernatant. Physicochemical analysis showed this antimicrobial component was not tolerant to high temperature and high and low acidity,and not sensitive to ultraviolet radiation,protease K,5 common metal ions and organic solvent. This component could be extracted by acid precipitation and methanol extraction. Single factor experiment showed this strain had the highest pathogen antagonistic activity when shake cultured at 28 ℃ and 180 r·min-1 for 3d on YPD medium inoculated at the rate of 7% and the initial pH7.

FAN Lanyan, HE Chunping, LIANG Yanqiong, ZHENG Xiaolan, WU Weihuai, LI Rui, ZHENG Fucong. Characteristics of the Antimicrobial Active Component of Bacillus subtilis Strain Czk1[J]. Journal of Tropical Biology, 2013, 4(1): 36-44,55. doi: 10.15886/j.cnki.rdswxb.2013.01.007
Citation: FAN Lanyan, HE Chunping, LIANG Yanqiong, ZHENG Xiaolan, WU Weihuai, LI Rui, ZHENG Fucong. Characteristics of the Antimicrobial Active Component of Bacillus subtilis Strain Czk1[J]. Journal of Tropical Biology, 2013, 4(1): 36-44,55. doi: 10.15886/j.cnki.rdswxb.2013.01.007
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