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Volume 4 Issue 1
Jan.  2013
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(1): 25-30

ZHAI Liyan, LI Juanling, LIU Guomin, XIE Jun, LUO Yiqi, CHENG Shanhan, PAN Xuefeng. RAPD Markers Linked to the Resistance Gene of Ilex kudingcha Against Colletotrichum illics-kudingchae[J]. Journal of Tropical Biology, 2013, 4(1): 25-30. doi: 10.15886/j.cnki.rdswxb.2013.01.006
Citation: ZHAI Liyan, LI Juanling, LIU Guomin, XIE Jun, LUO Yiqi, CHENG Shanhan, PAN Xuefeng. RAPD Markers Linked to the Resistance Gene of Ilex kudingcha Against Colletotrichum illics-kudingchae[J]. Journal of Tropical Biology, 2013, 4(1): 25-30. doi: 10.15886/j.cnki.rdswxb.2013.01.006
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RAPD Markers Linked to the Resistance Gene of Ilex kudingcha Against Colletotrichum illics-kudingchae

doi: 10.15886/j.cnki.rdswxb.2013.01.006

  • Kudingcha Research Institute, Hainan University, Haikou 570228, China

  • Received Date: 2012-05-17
  • In order to develop specific SCAR markers closely linked to the gene resistant to Colletotrichum illics-kudingchae G. M. Liu et Z. Y. Zhang,germplasm materials of Ilex kudingcha with high resistance and high susceptibility to C. illics-kudingchae were collected for RAPD analysis with 92 random primers,from which a few specific RAPD markers closely linked to the resistance gene were amplified. These markers included the specific fragments: S69-300,S227-320,S227-2000 and S247-400. These specific fragments will be retrieved,amplified and sequenced. The specific RAPD fragment sequencing results can be used for development of SCAR markers linked to the resistance gene against C. illics-kudingchae.
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RAPD Markers Linked to the Resistance Gene of Ilex kudingcha Against Colletotrichum illics-kudingchae

doi: 10.15886/j.cnki.rdswxb.2013.01.006
  • Kudingcha Research Institute, Hainan University, Haikou 570228, China
  • Received Date: 2012-05-17

Abstract: In order to develop specific SCAR markers closely linked to the gene resistant to Colletotrichum illics-kudingchae G. M. Liu et Z. Y. Zhang,germplasm materials of Ilex kudingcha with high resistance and high susceptibility to C. illics-kudingchae were collected for RAPD analysis with 92 random primers,from which a few specific RAPD markers closely linked to the resistance gene were amplified. These markers included the specific fragments: S69-300,S227-320,S227-2000 and S247-400. These specific fragments will be retrieved,amplified and sequenced. The specific RAPD fragment sequencing results can be used for development of SCAR markers linked to the resistance gene against C. illics-kudingchae.

ZHAI Liyan, LI Juanling, LIU Guomin, XIE Jun, LUO Yiqi, CHENG Shanhan, PAN Xuefeng. RAPD Markers Linked to the Resistance Gene of Ilex kudingcha Against Colletotrichum illics-kudingchae[J]. Journal of Tropical Biology, 2013, 4(1): 25-30. doi: 10.15886/j.cnki.rdswxb.2013.01.006
Citation: ZHAI Liyan, LI Juanling, LIU Guomin, XIE Jun, LUO Yiqi, CHENG Shanhan, PAN Xuefeng. RAPD Markers Linked to the Resistance Gene of Ilex kudingcha Against Colletotrichum illics-kudingchae[J]. Journal of Tropical Biology, 2013, 4(1): 25-30. doi: 10.15886/j.cnki.rdswxb.2013.01.006
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