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Volume 4 Issue 1
Jan.  2013
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Article Navigation >  Journal of Tropical Biology  > 2013  >  4(1): 31-35

ZI Liang, HONG Hao, ZHAI Jinling, MA Sui, HUANG Xi. Construction of Bait Vector for NiNJA Gene in Arabidopsis and Screening the Interacted Proteins Using Yeast Two-hybrid[J]. Journal of Tropical Biology, 2013, 4(1): 31-35. doi: 10.15886/j.cnki.rdswxb.2013.01.002
Citation: ZI Liang, HONG Hao, ZHAI Jinling, MA Sui, HUANG Xi. Construction of Bait Vector for NiNJA Gene in Arabidopsis and Screening the Interacted Proteins Using Yeast Two-hybrid[J]. Journal of Tropical Biology, 2013, 4(1): 31-35. doi: 10.15886/j.cnki.rdswxb.2013.01.002
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Construction of Bait Vector for NiNJA Gene in Arabidopsis and Screening the Interacted Proteins Using Yeast Two-hybrid

doi: 10.15886/j.cnki.rdswxb.2013.01.002

  • 1. Key Laboratory of Tropic Biological Resources of Ministry of Education/Institute of Bio-Science and Technology, College of Agromy, Hainan University, Haikou 570228, China

  • Received Date: 2013-03-10
  • JAZ proteins are important negative factors for jasmonic acid （ JA） signaling pathways in Arabidopsis thaliana. NiNJA interacted with JAZ proteins to inhibit the transcription activity of JA-related transcription factors. NiNJA is a key factor that connected the JAZs and JA-related transcription factors. In order to screen NiNJA interacted proteins,NiNJA full-length cDNA sequence was PCR amplified inserted into pGBKT7 vector to construct a bait vector for yeast two-hybrid system. Furthermore,the vector was transformed into Y2H Gold strain. The result of autoactivation showed that pGBKT7-NiNJA vector had no autoactivation. Finally,the bait vector was used to screen the interacted proteins in "Mate ＆ PlateTM "Library and several potential interacted proteins were obtained. This work laid a foundation for further identification of NiNJA interacted proteins and related signaling pathway.
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Construction of Bait Vector for NiNJA Gene in Arabidopsis and Screening the Interacted Proteins Using Yeast Two-hybrid

doi: 10.15886/j.cnki.rdswxb.2013.01.002
  • 1. Key Laboratory of Tropic Biological Resources of Ministry of Education/Institute of Bio-Science and Technology, College of Agromy, Hainan University, Haikou 570228, China
  • Received Date: 2013-03-10

Abstract: JAZ proteins are important negative factors for jasmonic acid （ JA） signaling pathways in Arabidopsis thaliana. NiNJA interacted with JAZ proteins to inhibit the transcription activity of JA-related transcription factors. NiNJA is a key factor that connected the JAZs and JA-related transcription factors. In order to screen NiNJA interacted proteins,NiNJA full-length cDNA sequence was PCR amplified inserted into pGBKT7 vector to construct a bait vector for yeast two-hybrid system. Furthermore,the vector was transformed into Y2H Gold strain. The result of autoactivation showed that pGBKT7-NiNJA vector had no autoactivation. Finally,the bait vector was used to screen the interacted proteins in "Mate ＆ PlateTM "Library and several potential interacted proteins were obtained. This work laid a foundation for further identification of NiNJA interacted proteins and related signaling pathway.

ZI Liang, HONG Hao, ZHAI Jinling, MA Sui, HUANG Xi. Construction of Bait Vector for NiNJA Gene in Arabidopsis and Screening the Interacted Proteins Using Yeast Two-hybrid[J]. Journal of Tropical Biology, 2013, 4(1): 31-35. doi: 10.15886/j.cnki.rdswxb.2013.01.002
Citation: ZI Liang, HONG Hao, ZHAI Jinling, MA Sui, HUANG Xi. Construction of Bait Vector for NiNJA Gene in Arabidopsis and Screening the Interacted Proteins Using Yeast Two-hybrid[J]. Journal of Tropical Biology, 2013, 4(1): 31-35. doi: 10.15886/j.cnki.rdswxb.2013.01.002
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