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Volume 3 Issue 4
Dec.  2012
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Article Navigation >  Journal of Tropical Biology  > 2012  >  3(4): 379-383386

WANG Xuebing, YU Xudong, ZHU Huijun, WU Fanhua, HUANG Xiaofeng, HU Xinwen. Extraction of Genomic DNA of Neottopteris nidus and Optimization of the RAPD Reaction System[J]. Journal of Tropical Biology, 2012, 3(4): 379-383386. doi: 10.15886/j.cnki.rdswxb.2012.04.019
Citation: WANG Xuebing, YU Xudong, ZHU Huijun, WU Fanhua, HUANG Xiaofeng, HU Xinwen. Extraction of Genomic DNA of Neottopteris nidus and Optimization of the RAPD Reaction System[J]. Journal of Tropical Biology, 2012, 3(4): 379-383386. doi: 10.15886/j.cnki.rdswxb.2012.04.019
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Extraction of Genomic DNA of Neottopteris nidus and Optimization of the RAPD Reaction System

doi: 10.15886/j.cnki.rdswxb.2012.04.019

  • 1. College of Agronomy, Hainan University, Haikou 570228, China;

  • 2. Research and Development Center for Fine Chemicals/State Key Laboratory Breeding Base for Green Pesticide and Agricultural Bioengineering/Ministry of Education Key Laboratory, Guiyang, Guizhou 550000, China;

  • 3. Ministry of Education Key Laboratory for Conservation and Utilization of Tropical Crops Germplasm Resources, Haikou 570228, China;

  • 4. Dongguan Botanic Garden, Dongguan, Guangdong 523086, China

  • Received Date: 2012-11-10
  • In order to study the genitic diversity and genetic structure of Bird’s nest fern（Neottopteris nidus） populations,fresh tender fronds of the Bird’s nest fern were collected and resorted to genomic DNA extraction through optimized RAPD reaction system by using different concentrations of CTAB and NaCl,different volumes of template DNA and primer.The improved CTAB DNA extraction method was the best to extract the genomic DNA of the fronds of the Bird’s nest fern.The optimum reaction system（25 μL） for RAPD amplification of the Bird’s nest fern DNA was listed as follows:The template DNA（50 mg·L-1）1.5μL;the primer（1 mmol·L-1） 1.5 μL;2×EasyTaq PCR SuperMiX 12.5 μL.
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    [8] 刘建锋,肖文发.濒危植物崖柏遗传多样性的RAPD分析[J].江西农业大学学报,2008,30(1):68-72.
    [9] 王艇,苏应娟,欧阳蒲月,等.利用RAPD标记分析濒危植物白豆杉种群的遗传结构[J].生态学报,2006,26(7):2313-2316.
    [10] 张颖娟,王玉山.濒危灌木长叶红砂遗传多样性RAPD的分析[J].生物学杂志,2008,27(2):157-161.
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    [13] 周延清,杨清香,张改娜.生物遗传标记与应用[M].北京:化学工业出版社,2008:28-93.
    [14] 付再萍.狗脊蕨(Woodwardia japonica(L.f.)Sm)基因组DNA提取与随机扩增多态性DNA(RAPD)的优化[J].杭州师范学院学报:自然科学版,2007,6(2):129-132.
    [15] 沈洁,罗安才.提取蕨类植物DNA方法比较[J].安徽农业科学,2010,38(4):1738-1740.
    [16] 陈进明,王青峰.珍稀濒危蕨类植物东方水韭的遗传多样性[J].武汉植物学研究,2006,24(6):569-573.
    [17] 陈进明,刘星,王青峰.中国珍稀濒危特有蕨类植物——云贵水韭的遗传多样性[J].武汉大学学报:理学版,2005,51(6):767-770.
    [18] 陈进明,王晶苑,刘星.中华水韭遗传多样性的RAPD分析[J].生物多样性,2004(12):348-353.
    [19] 李先进,彭正松,张正英,等.四种药用蕨类DNA提取的比较研究[J].绵阳师范学院学报,2008,27(8):67-69.
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Extraction of Genomic DNA of Neottopteris nidus and Optimization of the RAPD Reaction System

doi: 10.15886/j.cnki.rdswxb.2012.04.019
  • 1. College of Agronomy, Hainan University, Haikou 570228, China;
  • 2. Research and Development Center for Fine Chemicals/State Key Laboratory Breeding Base for Green Pesticide and Agricultural Bioengineering/Ministry of Education Key Laboratory, Guiyang, Guizhou 550000, China;
  • 3. Ministry of Education Key Laboratory for Conservation and Utilization of Tropical Crops Germplasm Resources, Haikou 570228, China;
  • 4. Dongguan Botanic Garden, Dongguan, Guangdong 523086, China
  • Received Date: 2012-11-10

Abstract: In order to study the genitic diversity and genetic structure of Bird’s nest fern（Neottopteris nidus） populations,fresh tender fronds of the Bird’s nest fern were collected and resorted to genomic DNA extraction through optimized RAPD reaction system by using different concentrations of CTAB and NaCl,different volumes of template DNA and primer.The improved CTAB DNA extraction method was the best to extract the genomic DNA of the fronds of the Bird’s nest fern.The optimum reaction system（25 μL） for RAPD amplification of the Bird’s nest fern DNA was listed as follows:The template DNA（50 mg·L-1）1.5μL;the primer（1 mmol·L-1） 1.5 μL;2×EasyTaq PCR SuperMiX 12.5 μL.

WANG Xuebing, YU Xudong, ZHU Huijun, WU Fanhua, HUANG Xiaofeng, HU Xinwen. Extraction of Genomic DNA of Neottopteris nidus and Optimization of the RAPD Reaction System[J]. Journal of Tropical Biology, 2012, 3(4): 379-383386. doi: 10.15886/j.cnki.rdswxb.2012.04.019
Citation: WANG Xuebing, YU Xudong, ZHU Huijun, WU Fanhua, HUANG Xiaofeng, HU Xinwen. Extraction of Genomic DNA of Neottopteris nidus and Optimization of the RAPD Reaction System[J]. Journal of Tropical Biology, 2012, 3(4): 379-383386. doi: 10.15886/j.cnki.rdswxb.2012.04.019
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