Genetic Diversity of Banana Varieties Based on Genes from Chloroplast

PENG Tiecheng; XU Fang; LUO Jingyao; CHEN Yunyun; TANG Hua

doi:10.15886/j.cnki.rdswxb.2012.04.013

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Banana is the most important tropical fruit.In recent years the outbreaks of banana Fusarium wilt have great negative impact on banana production.The study of genetic diversity of banana will provide support to genetic improvement of banana for resistance to Fusarium wilt.Seven banana varieties were used as sequencing materials,and 2 non-coding chloroplast DNA sequences（Intron sequence of rpl16 gene and intergenic sequence of psaA-ycf3 genes） in these varieties were amplified by PCR,cloned by TA,sequenced and analysed by bioinformatics to study the genetic diversity of banana.Neighbor joining method was used to establish phylogenetic tree and relative genetic distance measured.Results showed that Baxi banana had different degrees of genetic diversity as against the other 6 wilt-resistant banana varieties but the difference was not significant.

Genetic Diversity of Banana Varieties Based on Genes from Chloroplast

1. Key Laboratory for Conservation and Utilization of Tropical Crops Germplasm Resources, Ministry of Education, Haikou 570228, China;

2. Hainan Key Laboratory for Sustainable Utilization of Tropical Bio-resources-State Key Laboratory Breeding Base, Hainan University, Haikou 570228, China;

3. College of Agronomy, Hainan University, Haikou 570228, China

Received Date: 2012-10-25

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Abstract: Banana is the most important tropical fruit.In recent years the outbreaks of banana Fusarium wilt have great negative impact on banana production.The study of genetic diversity of banana will provide support to genetic improvement of banana for resistance to Fusarium wilt.Seven banana varieties were used as sequencing materials,and 2 non-coding chloroplast DNA sequences（Intron sequence of rpl16 gene and intergenic sequence of psaA-ycf3 genes） in these varieties were amplified by PCR,cloned by TA,sequenced and analysed by bioinformatics to study the genetic diversity of banana.Neighbor joining method was used to establish phylogenetic tree and relative genetic distance measured.Results showed that Baxi banana had different degrees of genetic diversity as against the other 6 wilt-resistant banana varieties but the difference was not significant.

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Reference (7)

[1]	KAEMMER D,AFZA R,WEISING K,et al.Oligonucleotide and amplification fingerprinting of wild species and cultivars of banana(Musa spp.)[J].Biotechnology(N Y),1992,10(9):1030-1035.
[2]	DEASCENSAO A R,DUBERY I A.Panama disease:cell wall reinforcement in banana roots in response to elicitors from fusar-ium oxysporum f.sp.cubense Race Four[J].Phytopathology,2000,90(10):1173-1180.
[3]	陈厚彬,冯奇瑞,徐春香,等.抗枯萎病香蕉种质筛选[J].华南农业大学学报,2006(1):9-12.
[4]	严定平,柳晓磊,汤华.香蕉枯萎病及其抗病育种研究进展[J].海南大学学报:自然科学版,2008,26(3):290-295.
[5]	SWANGPOL S,VOLKAERT H,SOTTO R C,et al.Utility of selected non-coding chloroplast DNA sequences for lineage as-sessment of Musa interspecific hybrids[J].J Biochem Mol Biol,2007,40(4):577-587.
[6]	ZHANG W.Phylogeny of the grass family(Poaceae)from rpl16intron sequence data[J].Mol Phylogenet Evol,2000,15(1):135-146.
[7]	SUMMER H,PFANNSCHMIDT T,LINK G.Transcripts and sequence elements suggest differential promoter usage within the ycf3-psaAB gene cluster on mustard(Sinapis alba L.)chloroplast DNA[J].Curr Genet,2000,37(1):45-52.

[1]	KAEMMER D,AFZA R,WEISING K,et al.Oligonucleotide and amplification fingerprinting of wild species and cultivars of banana(Musa spp.)[J].Biotechnology(N Y),1992,10(9):1030-1035.
[2]	DEASCENSAO A R,DUBERY I A.Panama disease:cell wall reinforcement in banana roots in response to elicitors from fusar-ium oxysporum f.sp.cubense Race Four[J].Phytopathology,2000,90(10):1173-1180.
[3]	陈厚彬,冯奇瑞,徐春香,等.抗枯萎病香蕉种质筛选[J].华南农业大学学报,2006(1):9-12.
[4]	严定平,柳晓磊,汤华.香蕉枯萎病及其抗病育种研究进展[J].海南大学学报:自然科学版,2008,26(3):290-295.
[5]	SWANGPOL S,VOLKAERT H,SOTTO R C,et al.Utility of selected non-coding chloroplast DNA sequences for lineage as-sessment of Musa interspecific hybrids[J].J Biochem Mol Biol,2007,40(4):577-587.
[6]	ZHANG W.Phylogeny of the grass family(Poaceae)from rpl16intron sequence data[J].Mol Phylogenet Evol,2000,15(1):135-146.
[7]	SUMMER H,PFANNSCHMIDT T,LINK G.Transcripts and sequence elements suggest differential promoter usage within the ycf3-psaAB gene cluster on mustard(Sinapis alba L.)chloroplast DNA[J].Curr Genet,2000,37(1):45-52.

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Genetic Diversity of Banana Varieties Based on Genes from Chloroplast

doi: 10.15886/j.cnki.rdswxb.2012.04.013

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通讯作者: 陈斌, bchen63@163.com

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