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HUANG Qixing, GUO Anping, YANG Lifu, ZOU Zhi. In silico Identification and Bioinformatic Analysis of Castor Bean WRKY14 Gene[J]. Journal of Tropical Biology, 2012, 3(4): 305-313. doi: 10.15886/j.cnki.rdswxb.2012.04.005
Citation: HUANG Qixing, GUO Anping, YANG Lifu, ZOU Zhi. In silico Identification and Bioinformatic Analysis of Castor Bean WRKY14 Gene[J]. Journal of Tropical Biology, 2012, 3(4): 305-313. doi: 10.15886/j.cnki.rdswxb.2012.04.005

In silico Identification and Bioinformatic Analysis of Castor Bean WRKY14 Gene

doi: 10.15886/j.cnki.rdswxb.2012.04.005
  • Received Date: 2012-09-26
  • WRKY is a plant-specific transcription factor that could be a key regulator in the stress response processes of castor bean(Ricinus communis L.).Consulting the published castor bean genome information,we corrected some gene annotation errors and successfully obtained castor bean RcWRKY14 gene by In silico cloning.Bioinformatic analysis showed that RcWRKY14 contains three introns,encoding a peptide of 318 amino acids,and that it is nuclear localized.RcWRKY14 encodes an unstable and hydrophilic protein with a theoretical molecular weight of 35.49 kDa,isoelectric point of 8.64,total average hydrophilic of-0.803,instability coefficient(II) of 46.88.The protein contains one WRKY domain with a pattern of WRKYGQK_X_13_C_X_5_C_X_23_H_X_1_H_.The conservative intron is located between site 199(K amino acid residue) and site 200(V amino acid residue) in the WRKY domain of RcWRKY14.The protein could be classified into subclass a under class II.Further analysis showed that a variety of hormone and stress-responsive cis-acting regulatory elements were found in the promoter region of RcWRKY14.Gene expression analysis showed that RcWRKY14 was expressed in castor bean leaves,flowers,endosperms and seeds,with the highest expression level in flowers,the lower in seeds and leaves and the lowest in the endosperms.
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In silico Identification and Bioinformatic Analysis of Castor Bean WRKY14 Gene

doi: 10.15886/j.cnki.rdswxb.2012.04.005

Abstract: WRKY is a plant-specific transcription factor that could be a key regulator in the stress response processes of castor bean(Ricinus communis L.).Consulting the published castor bean genome information,we corrected some gene annotation errors and successfully obtained castor bean RcWRKY14 gene by In silico cloning.Bioinformatic analysis showed that RcWRKY14 contains three introns,encoding a peptide of 318 amino acids,and that it is nuclear localized.RcWRKY14 encodes an unstable and hydrophilic protein with a theoretical molecular weight of 35.49 kDa,isoelectric point of 8.64,total average hydrophilic of-0.803,instability coefficient(II) of 46.88.The protein contains one WRKY domain with a pattern of WRKYGQK_X_13_C_X_5_C_X_23_H_X_1_H_.The conservative intron is located between site 199(K amino acid residue) and site 200(V amino acid residue) in the WRKY domain of RcWRKY14.The protein could be classified into subclass a under class II.Further analysis showed that a variety of hormone and stress-responsive cis-acting regulatory elements were found in the promoter region of RcWRKY14.Gene expression analysis showed that RcWRKY14 was expressed in castor bean leaves,flowers,endosperms and seeds,with the highest expression level in flowers,the lower in seeds and leaves and the lowest in the endosperms.

HUANG Qixing, GUO Anping, YANG Lifu, ZOU Zhi. In silico Identification and Bioinformatic Analysis of Castor Bean WRKY14 Gene[J]. Journal of Tropical Biology, 2012, 3(4): 305-313. doi: 10.15886/j.cnki.rdswxb.2012.04.005
Citation: HUANG Qixing, GUO Anping, YANG Lifu, ZOU Zhi. In silico Identification and Bioinformatic Analysis of Castor Bean WRKY14 Gene[J]. Journal of Tropical Biology, 2012, 3(4): 305-313. doi: 10.15886/j.cnki.rdswxb.2012.04.005
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