HAN Ming, LI Yanping, PAN Xuefeng. In vitro Propagation of Cymbidium via Clustered Shoots[J]. Journal of Tropical Biology, 2012, 3(3): 261-266. doi: 10.15886/j.cnki.rdswxb.2012.03.015
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HAN Ming, LI Yanping, PAN Xuefeng. In vitro Propagation of Cymbidium via Clustered Shoots[J]. Journal of Tropical Biology, 2012, 3(3): 261-266. doi: 10.15886/j.cnki.rdswxb.2012.03.015
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In vitro Propagation of Cymbidium via Clustered Shoots
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1. Haikou Institute of Landscape Architecture, Haikou 570206, China;
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2. College of Agronomy, Hainan University, Haikou 570228, China
- Received Date: 2012-07-12
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Abstract
Lateral buds of Cymbidium were used as explants to induce to form clustered shoots or multiple shoot clumps for rapid propagation of Cymbidium. The multiple shoot clumps were proliferated much faster when cultured on 1/2MS+ 6-BA 3.5 mg·L-1+NAA 0.2 mg·L-1+10% coconut water （CW） （or 10% banana homogenate） medium, and proliferation could be done at least 9 times a year when a subculture cycle took 40 days to complete, or a lateral bud can produce 3.69（about 100 thousand） plants a year. Browning occurred occasionally in multiple shoot clumps during the subculture but did not give negative impact on the proliferation of the multiple shoot clumps. Addition of 10% coconut water （CW） （or 10% banana homogenate） into the bud proliferation medium gave a higher proliferation rate of multiple shoot clumps and improved their growth vigor. The test tube plantlets were rooted well with the rooting rate of 86.1% when cultured on the rooting medium of 1/2MS+NAA1.0 mg·L-1+1.0 g·L-1 AC+10% CW. The rooted plantlets had a survival rate of upto 92.5% when transferred and cultured on the sphagnum.
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References
|
[1]
|
许震寰,刘洪科.大花蕙兰研究进展[J].安徽农学通报,2008,14(19):116-119
,18. |
|
[2]
|
王丽艳,刘汉云.大花蕙兰离体培养和快速繁殖技术的研究[J].科技情报开发与经济,2007,17(8):146-147. |
|
[3]
|
朱根发,蒋明殿.大花蕙兰的组织培养和快速繁殖技术[J].广东农业科学,2004(4):119-121. |
|
[4]
|
杨录军,李晓青.大花蕙兰组培工厂化生产技术[J].北方园艺,2007(8):186-187. |
|
[5]
|
邹宗兰,秦庭豪,文颖,等.大花蕙兰工厂化繁殖技术研究[J].西南农业学报,2007,20(4):743-746. |
|
[6]
|
|
|
[7]
|
刘丽锋,张健.丛生芽-大花蕙兰快速繁殖新途径[J].四川农业大学学报,2004,22(2):150-152. |
|
[8]
|
刘荣维,梅庆超,崔元方,等.丛生芽-蝴蝶兰无性快速繁殖的新途径[J].热带作物学报,1993,14(2):105-107. |
|
[9]
|
曹孜义,刘国民.实用植物组织培养技术教程[M].兰州:甘肃科学技术出版社,1996:34-37,350-363. |
|
[10]
|
陈正华.木本植物组织培养及其应用[M].北京:高等教育出版社,1986:34-35. |
|
[11]
|
张树珍,曾宪松,张银东,等.大花蕙兰的组织培养[J].热带作物研究,1995(4):26-28. |
|
[12]
|
卢思聪.中国兰花与洋兰[M].北京:金盾出版社,1994:62-81. |
|
[13]
|
姬君兆,黄玲燕.花木栽培技术问答[M].北京:化学工业出版社,2000. |
|
[14]
|
潭文澄,戴策刚.观赏植物组织培养技术[M].北京:中国林业出版社,1991:60-65. |
|
[15]
|
吴伟怀,郑肖兰,郑服丛.香蕉组培苗变异的原因、防止及其利用研究综述[J].中国南方果树,2007,36(4):31-33. |
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