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Volume 3 Issue 3
Sep.  2012
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Article Navigation >  Journal of Tropical Biology  > 2012  >  3(3): 236-242

LIU Yan, LIU Sixin, LI Yongcheng, LI Congfa. Optimization of Homoharringtonine Fermentation Conditions for Alternaria tenuissima CH1307,an Endophytical Fungus of Cephalotaxus mannii Hook.f.[J]. Journal of Tropical Biology, 2012, 3(3): 236-242. doi: 10.15886/j.cnki.rdswxb.2012.03.003
Citation: LIU Yan, LIU Sixin, LI Yongcheng, LI Congfa. Optimization of Homoharringtonine Fermentation Conditions for Alternaria tenuissima CH1307,an Endophytical Fungus of Cephalotaxus mannii Hook.f.[J]. Journal of Tropical Biology, 2012, 3(3): 236-242. doi: 10.15886/j.cnki.rdswxb.2012.03.003
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Optimization of Homoharringtonine Fermentation Conditions for Alternaria tenuissima CH1307,an Endophytical Fungus of Cephalotaxus mannii Hook.f.

doi: 10.15886/j.cnki.rdswxb.2012.03.003

  • College of Food Science and Technology, Hainan University, Haikou, 570228, China

  • Received Date: 2012-05-07
  • Homoharringtonine （HHRT） was produced from Alternaria tenuissima CH1307, an endophytical fungus of Cephalotaxus mannii Hook.f through fermentation. Four factors such as the concentrations of glucose, magnesium sulfate and vitamin B compound and the rotating speed were analyzed by using response surface methodology to determine their effect on fermentation of the fungus CH1370. The best operating conditions resulted to be 32.022 6 g·L-1 for glucose , 0.096 6 g·L-1 for magnesium sulfate and 0.123 9 g·L-1 for vitamin B compound as well as 146.474 2 r·min-1 for the rotating speed, and 190.637 0 μg·L-1 of HHRT were produced from this fungus when the values of the four factors were entered into a formula established.
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Optimization of Homoharringtonine Fermentation Conditions for Alternaria tenuissima CH1307,an Endophytical Fungus of Cephalotaxus mannii Hook.f.

doi: 10.15886/j.cnki.rdswxb.2012.03.003
  • College of Food Science and Technology, Hainan University, Haikou, 570228, China
  • Received Date: 2012-05-07

Abstract: Homoharringtonine （HHRT） was produced from Alternaria tenuissima CH1307, an endophytical fungus of Cephalotaxus mannii Hook.f through fermentation. Four factors such as the concentrations of glucose, magnesium sulfate and vitamin B compound and the rotating speed were analyzed by using response surface methodology to determine their effect on fermentation of the fungus CH1370. The best operating conditions resulted to be 32.022 6 g·L-1 for glucose , 0.096 6 g·L-1 for magnesium sulfate and 0.123 9 g·L-1 for vitamin B compound as well as 146.474 2 r·min-1 for the rotating speed, and 190.637 0 μg·L-1 of HHRT were produced from this fungus when the values of the four factors were entered into a formula established.

LIU Yan, LIU Sixin, LI Yongcheng, LI Congfa. Optimization of Homoharringtonine Fermentation Conditions for Alternaria tenuissima CH1307,an Endophytical Fungus of Cephalotaxus mannii Hook.f.[J]. Journal of Tropical Biology, 2012, 3(3): 236-242. doi: 10.15886/j.cnki.rdswxb.2012.03.003
Citation: LIU Yan, LIU Sixin, LI Yongcheng, LI Congfa. Optimization of Homoharringtonine Fermentation Conditions for Alternaria tenuissima CH1307,an Endophytical Fungus of Cephalotaxus mannii Hook.f.[J]. Journal of Tropical Biology, 2012, 3(3): 236-242. doi: 10.15886/j.cnki.rdswxb.2012.03.003
shu

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