TANG Yi-li, XIE Xiu-chao, HONG Kui. Identification and evaluation of physiologically active root endophytic actinomycetes from mangrove plants in Hainan Province[J]. Journal of Tropical Biology, 2012, 3(1): 32-37,41. doi: 10.15886/j.cnki.rdswxb.2012.01.013
| Citation:
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TANG Yi-li, XIE Xiu-chao, HONG Kui. Identification and evaluation of physiologically active root endophytic actinomycetes from mangrove plants in Hainan Province[J]. Journal of Tropical Biology, 2012, 3(1): 32-37,41. doi: 10.15886/j.cnki.rdswxb.2012.01.013
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Identification and evaluation of physiologically active root endophytic actinomycetes from mangrove plants in Hainan Province
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1. College of Agronomy, Hainan University, Haikou 570228, China;
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2. College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723003, China;
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3. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
- Received Date: 2011-11-25
- Publish Date:
2012-03-25
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Abstract
A total of 138 root endophytic actinomycete strains were successfully isolated using 1/10ATCC 172 medium from the roots of 18 mangrove plants and 3 semi-mangrove plants in Hainan Province.Analysis of 16S rRNA gene sequences of these isolates showed that the isolates belonged to members of the genera Jishengella,Micromonospora and Streptomycetes.Bioactivity tests were carried out on all the isolated endophytic actinomycete fermentation liquids.Of all the isolates forty strains showed citotoxities to hepatocarcinoma cell line SMMC-7721 cell,and twenty seven strains were active against methicillin-resistant Staphylococcus aureus 9551（MRSA）.The strains isolated from the mangroves showed no bioactivity against Candida albicans ATCC 10231.Sodium chloride tolerance test for 88 isolates indicated that 77 isolates were halo-tolerant or halophilic,2 isolates of which exhibited the highest tolerance to ρ(NaCl）=150 g·L-1 and 54 isolates grew well on the medium containing ρ(NaCl）=33 g·L-1.
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