Induction of banana <i>Fusarium</i> Wilt disease with biocontrol bacteria and pathogen by inoculation

XUE Yu-xiao; JIA Hui-sheng; WANG Guo-fen; LIU Lei; HUANG Jun-sheng

doi:10.15886/j.cnki.rdswxb.2012.01.007

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Banana vitroplants of 4—5 leaves were inoculated in different ways with two biocontrol bacteria Bacillus subtilis strains A_5-6 and C_10-1 and the banana Fusarium wilt pathogen（Foc4） to observe their changes of polyphenol oxidase（PPO）,peroxidase（POD） and phenylalanine ammonialyase（PAL） activities in leaves,which are related to plant tolerance to the disease.The PPO,POD and PAL activities in the leaves of the banana plants were determined by using the physiological and biochemical methods.The banana plants inoculated with the strains A_5-6 and C_10-1 separately showed higher leaf PPO,POD and PAL activities than those inoculated with the sterile water control,and the banana plants inoculated with mixture of the biocontrol bacteria and Foc4 pathogen had higher leaf PPO,POD and PAL activities than those inoculated with Foc4 alone.

Induction of banana Fusarium Wilt disease with biocontrol bacteria and pathogen by inoculation

1. College of Agronomy, Hainan University, Haikou 570228, China;

2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Received Date: 2011-12-18

Publish Date: 2012-03-25

Key words:

Abstract: Banana vitroplants of 4—5 leaves were inoculated in different ways with two biocontrol bacteria Bacillus subtilis strains A_5-6 and C_10-1 and the banana Fusarium wilt pathogen（Foc4） to observe their changes of polyphenol oxidase（PPO）,peroxidase（POD） and phenylalanine ammonialyase（PAL） activities in leaves,which are related to plant tolerance to the disease.The PPO,POD and PAL activities in the leaves of the banana plants were determined by using the physiological and biochemical methods.The banana plants inoculated with the strains A_5-6 and C_10-1 separately showed higher leaf PPO,POD and PAL activities than those inoculated with the sterile water control,and the banana plants inoculated with mixture of the biocontrol bacteria and Foc4 pathogen had higher leaf PPO,POD and PAL activities than those inoculated with Foc4 alone.

HTML

Reference (18)

[1]	PLOETZ R C.Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f.sp.Cubense[J].Phytopathology2006,96(6):653-656.
[2]	王振中.香蕉枯萎病及其防治研究进展[J].植物检疫,2006(3):198-200.
[3]	杨秀娟,杜宜新,甘林,等.香蕉枯萎病生物防治和抗病育种研究进展[J].中国果树,2008(6):42-44.
[4]	孙丽华,宋刚,云兴福.黄瓜霜霉病诱导抗性的研究进展[J].内蒙古农业大学学报,2007,28(3):306-308.
[5]	郑莉,梁建根,施跃峰.生防菌ZJH-10对黄瓜灰霉病诱导抗性的研究[J].中国农学通报,2009,25(3):197-201.
[6]	陈志谊,许志刚,陆凡,等.拮抗细菌B-196对水稻植株的抗性诱导作用[J].西南农业学报,2001,14(2):44-48.
[7]	ZHAO H C,ZHAO H,WANG B C,et al.Effect of local stress induction on resistance-related enzymes in cucumber seeding[J].Colloids Surf B Biointerfaces.2005,43(1):37-42.
[8]	PELLEGRINI L,ROHFRITSEH O,FRITIG B,et al.Phenylalanine ammonialyase in tobacco[J].Plant Physiology,1994,106(3):877-886.
[9]	余叔文,汤章城.植物生理与分子生物学[M].北京:科学出版社,2001:770-807.
[10]	RAY H,DOUCHES D S,HAMMERSCHMIDT R.Transformation of potato with cucumber peroxidase:Expression and dis-ease response[J].Physiological and Molecular Plant Pathology,1998,53:93-103.
[11]	KAVINO M,KUMAR N,DAMODARAN T,et al.Biochemical markers as a useful tool for the early identification of Fusari-um oxysporum f.sp.Cubense,race1resistance banana clones[J].Archives of Phytopathology and Plant Protection,2009,42(11):1069-1078.
[12]	陈芳,慕小倩,梁宗锁,等.水杨酸对附子叶斑病的诱导抗性及作用机理研究[J].西北农业学报,2007,16(2):245-249.
[13]	马春红,李运朝,董文琦,等.低浓度玉米小斑病菌T毒素对玉米叶片苯丙氨酸解氨酶活性的诱导研究[J].华北农学报,2007,22(1):40-43.
[14]	游春平,肖爱萍,傅志岸,等.拮抗细菌对香蕉枯萎病的防治效果[J].仲恺农业技术学院学报,2005,18(4):16-20.
[15]	杨述省.生防菌JK-2粗霉素活性物质分析及对香蕉枯萎病诱导抗性的研究[D].福州:福建农林大学,2009.
[16]	牛晓磊.辣椒疫病生防菌剂作用效果及其诱导抗性研究[D].杨凌:西北农林科技大学,2005.
[17]	董玉红.蔬菜抗炭疽病诱导物的筛选及诱导抗性生化机理研究[D].南京:南京农业大学,2000.
[18]	KOOEPPER J W,TUZUN S,KUC J A.Proposed definitions related to induced disease resistance[J].Biocontrol Science and Technology,1992,2(4):349-351.

Message Board

Induction of banana Fusarium Wilt disease with biocontrol bacteria and pathogen by inoculation

doi: 10.15886/j.cnki.rdswxb.2012.01.007

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Related

Induction of banana Fusarium Wilt disease with biocontrol bacteria and pathogen by inoculation

doi: 10.15886/j.cnki.rdswxb.2012.01.007

1. College of Agronomy, Hainan University, Haikou 570228, China;

2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

HTML

Catalog

Message Board

Induction of banana Fusarium Wilt disease with biocontrol bacteria and pathogen by inoculation

doi: 10.15886/j.cnki.rdswxb.2012.01.007

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Related

Induction of banana Fusarium Wilt disease with biocontrol bacteria and pathogen by inoculation

doi: 10.15886/j.cnki.rdswxb.2012.01.007

1. College of Agronomy, Hainan University, Haikou 570228, China; 2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

HTML

Catalog

Export File

Citation

Format

Content

1. College of Agronomy, Hainan University, Haikou 570228, China;

2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China