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Volume 2 Issue 3
Sep.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(3): 226-229

ZHANG Yingxia, WEI Shuangshuang, LU Lu, CHEN Congwei, CHI Tingting, AN Tianchen, GUAN Huai, MAN Churiga. Purification and Characteristic of Albumin from Monopterus albus Skin[J]. Journal of Tropical Biology, 2011, 2(3): 226-229. doi: 10.15886/j.cnki.rdswxb.2011.03.022
Citation: ZHANG Yingxia, WEI Shuangshuang, LU Lu, CHEN Congwei, CHI Tingting, AN Tianchen, GUAN Huai, MAN Churiga. Purification and Characteristic of Albumin from Monopterus albus Skin[J]. Journal of Tropical Biology, 2011, 2(3): 226-229. doi: 10.15886/j.cnki.rdswxb.2011.03.022
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Purification and Characteristic of Albumin from Monopterus albus Skin

doi: 10.15886/j.cnki.rdswxb.2011.03.022

  • 1. Key Laboratory of Tropic Biological Resources, Minister of Education, Hainan University, Haikou 570228, China;

  • 2. Haikou VTI Biological Institute, Haikou 570228, China

  • Received Date: 2011-09-08
  • The anion exchange chromatography and gel filtration chromatography were performed and skin albumin of Monopterus albus was obtained. The protein is a single chain protein with molecular weigh of 67×103, and named MAA-skin. MAA-skin was determined as a glycoprotein by Sciff’s agent. The N-terminal amino acid sequence is GHVKW. The purified MAA-serum was used to immunize rabbits to prepare polyclonal antibody. ELISA data demonstrated that the antibody titer of the MAA-skin was up to 1∶ 10 000. Western blot results indicated that the polyclonal antibodies raised against MAA-serum strongly cross-reacted with both MAA-serum and MAA-skin with a compatible grade. The distribution of frog albumin in frog skin suggested that it plays important roles in skin physiological functions, such as water salt metabolism, osmotic pressure maintaining and substance transporting, etc.
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Purification and Characteristic of Albumin from Monopterus albus Skin

doi: 10.15886/j.cnki.rdswxb.2011.03.022
  • 1. Key Laboratory of Tropic Biological Resources, Minister of Education, Hainan University, Haikou 570228, China;
  • 2. Haikou VTI Biological Institute, Haikou 570228, China
  • Received Date: 2011-09-08

Abstract: The anion exchange chromatography and gel filtration chromatography were performed and skin albumin of Monopterus albus was obtained. The protein is a single chain protein with molecular weigh of 67×103, and named MAA-skin. MAA-skin was determined as a glycoprotein by Sciff’s agent. The N-terminal amino acid sequence is GHVKW. The purified MAA-serum was used to immunize rabbits to prepare polyclonal antibody. ELISA data demonstrated that the antibody titer of the MAA-skin was up to 1∶ 10 000. Western blot results indicated that the polyclonal antibodies raised against MAA-serum strongly cross-reacted with both MAA-serum and MAA-skin with a compatible grade. The distribution of frog albumin in frog skin suggested that it plays important roles in skin physiological functions, such as water salt metabolism, osmotic pressure maintaining and substance transporting, etc.

ZHANG Yingxia, WEI Shuangshuang, LU Lu, CHEN Congwei, CHI Tingting, AN Tianchen, GUAN Huai, MAN Churiga. Purification and Characteristic of Albumin from Monopterus albus Skin[J]. Journal of Tropical Biology, 2011, 2(3): 226-229. doi: 10.15886/j.cnki.rdswxb.2011.03.022
Citation: ZHANG Yingxia, WEI Shuangshuang, LU Lu, CHEN Congwei, CHI Tingting, AN Tianchen, GUAN Huai, MAN Churiga. Purification and Characteristic of Albumin from Monopterus albus Skin[J]. Journal of Tropical Biology, 2011, 2(3): 226-229. doi: 10.15886/j.cnki.rdswxb.2011.03.022
shu

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