Cloning of Ascorbate Peroxidase Gene HbAPX in Hevea brasiliensis and Its Response to Salicylic Acid

Key words: 
• Hevea brasiliensis /
• Ascorbate peroxidase（APX） /
• SA response

Abstract: Based on the sequencing results of RNA-Seq analysis of Hevea brasiliensis and the amino acid sequence of APX1 in Arabidopsis thaliana, the specific primers were designed and RT-PCR were performed to amplify the APX cDNA of Hevea brasiliensis（HbAPX）. The results showed that the open reading frame of HbAPX is 858bp encoding 285 amino acids with a conserved ascorbate peroxidase domain; the amino acid sequence of the HbAPX has 90% homology with APX from Jatropha curcas, and the homology is more than 80% with APX from Populus tomentosa, Gossypium hirsutum, Arabidopsis thaliana, Oryza sativa; the phylogenetic analysis data indicated that HbAPX belong to peroxisome type APX; the quantitative analysis data suggested that the expression of HbAPX was induced by SA, which is probably involved in system acquired resistance and hypersensitivity reaction in rubber.