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Volume 2 Issue 2
Jun.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(2): 123-128

LUO Jian, HUANG Zongwen, CHEN Guohua, WANG Jun, YANG Wei, GUO Renxiang. Microsatellite Analyses of Wild Population and Cultivation Artificial Propagation Population in Orange-Spotted Grouper （Epinephelus coioides） in the South China Sea[J]. Journal of Tropical Biology, 2011, 2(2): 123-128. doi: 10.15886/j.cnki.rdswxb.2011.02.019
Citation: LUO Jian, HUANG Zongwen, CHEN Guohua, WANG Jun, YANG Wei, GUO Renxiang. Microsatellite Analyses of Wild Population and Cultivation Artificial Propagation Population in Orange-Spotted Grouper （Epinephelus coioides） in the South China Sea[J]. Journal of Tropical Biology, 2011, 2(2): 123-128. doi: 10.15886/j.cnki.rdswxb.2011.02.019
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Microsatellite Analyses of Wild Population and Cultivation Artificial Propagation Population in Orange-Spotted Grouper （Epinephelus coioides） in the South China Sea

doi: 10.15886/j.cnki.rdswxb.2011.02.019

  • College of Ocean, Hainan University;Key Laboratory of Tropic Biological Resources of Ministry of Education, Hainan University, Haikou 570228, China

  • Received Date: 2011-04-24
  • Microsatellite makers were used to evaluate genetic variations of Orange-Spotted Grouper（Epinephelus coioides） in the South China Sea wild population and the Hainan cultivation artificial propagation population,and 48 individuals in each stock were assessed at 19 SSR loci.The result showed that the wild population（WP） and cultivation artificial propagation population（CP）,the number of effective alleles（Ne） were 1.135 0—4.516 2 and 1.00 0—3.982 7 respectively;values of mean observed heterozygosity（Ho） were 0.7061 and 0.687 5 respectively;expected heterozygosity（He） was 0.480 3 and 0.421 5 respectively;polymorphic information content（PIC） were 0.547 5 and 0.493 0 respectively;genetic similarity coefficient（Gst）,genetic differentiation coefficient（Fst） and genetic distance（D） between WP and CP were 0.979 4,0.015 7 and 0.020 8 respectively.These genetic parameters suggested that the genetic variations in the South China Sea wild population of E.coioides were well conserved and the genetic differentiation and degradation were not obvious in Hainan cultivation artificial propagation population.
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Microsatellite Analyses of Wild Population and Cultivation Artificial Propagation Population in Orange-Spotted Grouper （Epinephelus coioides） in the South China Sea

doi: 10.15886/j.cnki.rdswxb.2011.02.019
  • College of Ocean, Hainan University;Key Laboratory of Tropic Biological Resources of Ministry of Education, Hainan University, Haikou 570228, China
  • Received Date: 2011-04-24

Abstract: Microsatellite makers were used to evaluate genetic variations of Orange-Spotted Grouper（Epinephelus coioides） in the South China Sea wild population and the Hainan cultivation artificial propagation population,and 48 individuals in each stock were assessed at 19 SSR loci.The result showed that the wild population（WP） and cultivation artificial propagation population（CP）,the number of effective alleles（Ne） were 1.135 0—4.516 2 and 1.00 0—3.982 7 respectively;values of mean observed heterozygosity（Ho） were 0.7061 and 0.687 5 respectively;expected heterozygosity（He） was 0.480 3 and 0.421 5 respectively;polymorphic information content（PIC） were 0.547 5 and 0.493 0 respectively;genetic similarity coefficient（Gst）,genetic differentiation coefficient（Fst） and genetic distance（D） between WP and CP were 0.979 4,0.015 7 and 0.020 8 respectively.These genetic parameters suggested that the genetic variations in the South China Sea wild population of E.coioides were well conserved and the genetic differentiation and degradation were not obvious in Hainan cultivation artificial propagation population.

LUO Jian, HUANG Zongwen, CHEN Guohua, WANG Jun, YANG Wei, GUO Renxiang. Microsatellite Analyses of Wild Population and Cultivation Artificial Propagation Population in Orange-Spotted Grouper （Epinephelus coioides） in the South China Sea[J]. Journal of Tropical Biology, 2011, 2(2): 123-128. doi: 10.15886/j.cnki.rdswxb.2011.02.019
Citation: LUO Jian, HUANG Zongwen, CHEN Guohua, WANG Jun, YANG Wei, GUO Renxiang. Microsatellite Analyses of Wild Population and Cultivation Artificial Propagation Population in Orange-Spotted Grouper （Epinephelus coioides） in the South China Sea[J]. Journal of Tropical Biology, 2011, 2(2): 123-128. doi: 10.15886/j.cnki.rdswxb.2011.02.019
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