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Volume 2 Issue 2
Jun.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(2): 107-112

ZHANG Kun, XU Li, CONG Hanqing, DING Jing, HAO Honggang, LI Zhiying. Cloning and Sequencing of AfMKK1,A Novel MAPKK Gene Induced by Ethylene in Aechmea fasciata[J]. Journal of Tropical Biology, 2011, 2(2): 107-112. doi: 10.15886/j.cnki.rdswxb.2011.02.013
Citation: ZHANG Kun, XU Li, CONG Hanqing, DING Jing, HAO Honggang, LI Zhiying. Cloning and Sequencing of AfMKK1,A Novel MAPKK Gene Induced by Ethylene in Aechmea fasciata[J]. Journal of Tropical Biology, 2011, 2(2): 107-112. doi: 10.15886/j.cnki.rdswxb.2011.02.013
shu

Cloning and Sequencing of AfMKK1,A Novel MAPKK Gene Induced by Ethylene in Aechmea fasciata

doi: 10.15886/j.cnki.rdswxb.2011.02.013

  • 1. College of Agriculture, Hainan University, Haikou 570228, China;

  • 2. Tropical crops genetic resources institute, Chinese Academy of Tropical Agriculture Sciences, Danzhou 571737, China

  • Received Date: 2011-05-03
  • A cDNA fragments homologue of the known plant mitogen activated protein kinase（MAPKK,MKK,MEK） was selected by the suppression subtractive hybridization（SSH） library,and which can be induced by ethylene,and Rapid Amplification of cDNA Ends（RACE） were used to obtain the full length cDNA.Bioinformatic analysis results suggested that the gene belongs to a subfamily of plant MAPKK with a conserved region phosphorylation consensus sequence S/T*****S/T.Semi-quantitative RT-PCR results indicated that the expression of this gene increased after ethylene treatment,which suggested it may play an important role in the ethylene induction pathway.
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Cloning and Sequencing of AfMKK1,A Novel MAPKK Gene Induced by Ethylene in Aechmea fasciata

doi: 10.15886/j.cnki.rdswxb.2011.02.013
  • 1. College of Agriculture, Hainan University, Haikou 570228, China;
  • 2. Tropical crops genetic resources institute, Chinese Academy of Tropical Agriculture Sciences, Danzhou 571737, China
  • Received Date: 2011-05-03

Abstract: A cDNA fragments homologue of the known plant mitogen activated protein kinase（MAPKK,MKK,MEK） was selected by the suppression subtractive hybridization（SSH） library,and which can be induced by ethylene,and Rapid Amplification of cDNA Ends（RACE） were used to obtain the full length cDNA.Bioinformatic analysis results suggested that the gene belongs to a subfamily of plant MAPKK with a conserved region phosphorylation consensus sequence S/T*****S/T.Semi-quantitative RT-PCR results indicated that the expression of this gene increased after ethylene treatment,which suggested it may play an important role in the ethylene induction pathway.

ZHANG Kun, XU Li, CONG Hanqing, DING Jing, HAO Honggang, LI Zhiying. Cloning and Sequencing of AfMKK1,A Novel MAPKK Gene Induced by Ethylene in Aechmea fasciata[J]. Journal of Tropical Biology, 2011, 2(2): 107-112. doi: 10.15886/j.cnki.rdswxb.2011.02.013
Citation: ZHANG Kun, XU Li, CONG Hanqing, DING Jing, HAO Honggang, LI Zhiying. Cloning and Sequencing of AfMKK1,A Novel MAPKK Gene Induced by Ethylene in Aechmea fasciata[J]. Journal of Tropical Biology, 2011, 2(2): 107-112. doi: 10.15886/j.cnki.rdswxb.2011.02.013
shu

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