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Volume 2 Issue 2
Jun.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(2): 133-137

ZHI Lianteng, ZHAO Qian, AO Guangming, YU Jingjuan. Cloning of Fiber-specific Promoter LTP3 from Color Cotton （Gossyium hirhsutum） and Its Expression Specificity in Tobacco[J]. Journal of Tropical Biology, 2011, 2(2): 133-137. doi: 10.15886/j.cnki.rdswxb.2011.02.010
Citation: ZHI Lianteng, ZHAO Qian, AO Guangming, YU Jingjuan. Cloning of Fiber-specific Promoter LTP3 from Color Cotton （Gossyium hirhsutum） and Its Expression Specificity in Tobacco[J]. Journal of Tropical Biology, 2011, 2(2): 133-137. doi: 10.15886/j.cnki.rdswxb.2011.02.010
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Cloning of Fiber-specific Promoter LTP3 from Color Cotton （Gossyium hirhsutum） and Its Expression Specificity in Tobacco

doi: 10.15886/j.cnki.rdswxb.2011.02.010

  • College of Biological Sciences, State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100083, China

  • Received Date: 2011-06-05
  • PCR were performed to clone LTP3 promoter from color cotton.Sequencing analysis results indicated that it contained 1 548 nt;the homology is 99% compared with the LTP3 reported by Liu,and only 4 nucleotides were changed.The LTP3（1548bp） promoter was fused with the E.coli.β-glucuronidase gene（GUS） and transformed into tobacco.The results suggested that GUS expressions under control of LTP3 promoter were detected only in the trichomes of transgenic tobacco leaves,while the positive control,contains Cauliflower mosaic Virus（CaMV） 35S promoter and GUS gene,was constitutively expressed,and there are no GUS expression in the negative control（untransformation plants）.The results indicated that LTP3 promoter can be used to express the heterologous genes in tobacco leaves.
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Cloning of Fiber-specific Promoter LTP3 from Color Cotton （Gossyium hirhsutum） and Its Expression Specificity in Tobacco

doi: 10.15886/j.cnki.rdswxb.2011.02.010
  • College of Biological Sciences, State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100083, China
  • Received Date: 2011-06-05

Abstract: PCR were performed to clone LTP3 promoter from color cotton.Sequencing analysis results indicated that it contained 1 548 nt;the homology is 99% compared with the LTP3 reported by Liu,and only 4 nucleotides were changed.The LTP3（1548bp） promoter was fused with the E.coli.β-glucuronidase gene（GUS） and transformed into tobacco.The results suggested that GUS expressions under control of LTP3 promoter were detected only in the trichomes of transgenic tobacco leaves,while the positive control,contains Cauliflower mosaic Virus（CaMV） 35S promoter and GUS gene,was constitutively expressed,and there are no GUS expression in the negative control（untransformation plants）.The results indicated that LTP3 promoter can be used to express the heterologous genes in tobacco leaves.

ZHI Lianteng, ZHAO Qian, AO Guangming, YU Jingjuan. Cloning of Fiber-specific Promoter LTP3 from Color Cotton （Gossyium hirhsutum） and Its Expression Specificity in Tobacco[J]. Journal of Tropical Biology, 2011, 2(2): 133-137. doi: 10.15886/j.cnki.rdswxb.2011.02.010
Citation: ZHI Lianteng, ZHAO Qian, AO Guangming, YU Jingjuan. Cloning of Fiber-specific Promoter LTP3 from Color Cotton （Gossyium hirhsutum） and Its Expression Specificity in Tobacco[J]. Journal of Tropical Biology, 2011, 2(2): 133-137. doi: 10.15886/j.cnki.rdswxb.2011.02.010
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