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Volume 2 Issue 1
Mar.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(1): 14-20

YU Wenyuan, WANG Shifeng, XIE Zhenyu, CHEN Zhao, ZHOU Yongcan. Optimization of the Preparation Methods for Antimicrobial Peptides from Pinctada martensii[J]. Journal of Tropical Biology, 2011, 2(1): 14-20. doi: 10.15886/j.cnki.rdswxb.2011.01.016
Citation: YU Wenyuan, WANG Shifeng, XIE Zhenyu, CHEN Zhao, ZHOU Yongcan. Optimization of the Preparation Methods for Antimicrobial Peptides from Pinctada martensii[J]. Journal of Tropical Biology, 2011, 2(1): 14-20. doi: 10.15886/j.cnki.rdswxb.2011.01.016
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Optimization of the Preparation Methods for Antimicrobial Peptides from Pinctada martensii

doi: 10.15886/j.cnki.rdswxb.2011.01.016

  • Key Laboratory of Tropical Aquatic Biotechnology of Hainan Province; College of Marine Sciences, Hainan University, Haikou 570228, China

  • Received Date: 2011-01-29
  • The different processing methods were performed to extract the crude antimicrobial peptides extraction from the mantle and gill tissues of Pincatda martensii,and the activities of crude extractions were compared.The result suggested that the activities of crude extractions were not changed when Pincatda martensii was induced by Streptococcus agalactiae with 1.0×106 cfu·mL-1 for 14 h;the antibacterial activity of crude extractions homogenated by IKAT-18 homogenate machine was higher than that by the tissue grinder and the agate mortar;the antibacterial activity against Vibrio alginolyticus was significantly improved when the extraction was heated at 100 ℃ for 10 min;and the antibacterial activity against V.alginolyticus was significantly reduced when the crude extractions of the mantle tissue from P.martensii was frozen and thawed repeatedly.
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Optimization of the Preparation Methods for Antimicrobial Peptides from Pinctada martensii

doi: 10.15886/j.cnki.rdswxb.2011.01.016
  • Key Laboratory of Tropical Aquatic Biotechnology of Hainan Province; College of Marine Sciences, Hainan University, Haikou 570228, China
  • Received Date: 2011-01-29

Abstract: The different processing methods were performed to extract the crude antimicrobial peptides extraction from the mantle and gill tissues of Pincatda martensii,and the activities of crude extractions were compared.The result suggested that the activities of crude extractions were not changed when Pincatda martensii was induced by Streptococcus agalactiae with 1.0×106 cfu·mL-1 for 14 h;the antibacterial activity of crude extractions homogenated by IKAT-18 homogenate machine was higher than that by the tissue grinder and the agate mortar;the antibacterial activity against Vibrio alginolyticus was significantly improved when the extraction was heated at 100 ℃ for 10 min;and the antibacterial activity against V.alginolyticus was significantly reduced when the crude extractions of the mantle tissue from P.martensii was frozen and thawed repeatedly.

YU Wenyuan, WANG Shifeng, XIE Zhenyu, CHEN Zhao, ZHOU Yongcan. Optimization of the Preparation Methods for Antimicrobial Peptides from Pinctada martensii[J]. Journal of Tropical Biology, 2011, 2(1): 14-20. doi: 10.15886/j.cnki.rdswxb.2011.01.016
Citation: YU Wenyuan, WANG Shifeng, XIE Zhenyu, CHEN Zhao, ZHOU Yongcan. Optimization of the Preparation Methods for Antimicrobial Peptides from Pinctada martensii[J]. Journal of Tropical Biology, 2011, 2(1): 14-20. doi: 10.15886/j.cnki.rdswxb.2011.01.016
shu

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